PMID- 22231286
OWN - NLM
STAT- MEDLINE
DCOM- 20120917
LR  - 20211021
IS  - 1572-8781 (Electronic)
IS  - 1387-2176 (Print)
IS  - 1387-2176 (Linking)
VI  - 14
IP  - 3
DP  - 2012 Jun
TI  - Microfluidic extraction and stretching of chromosomal DNA from single cell nuclei 
      for DNA fluorescence in situ hybridization.
PG  - 443-51
LID - 10.1007/s10544-011-9621-8 [doi]
AB  - We have developed a novel method for genetic characterization of single cells by 
      integrating microfluidic stretching of chromosomal DNA and fiber fluorescence in 
      situ hybridization (FISH). In this method, individually isolated cell nuclei were 
      immobilized in a microchannel. Chromosomal DNA was released from the nuclei and 
      stretched by a pressure-driven flow. We analyzed and optimized flow conditions to 
      generate a millimeter-long band of stretched DNA from each nucleus. Telomere 
      fiber FISH was successfully performed on the stretched chromosomal DNA. 
      Individual telomere fiber FISH signals from single cells could be resolved and 
      their lengths measured, demonstrating the ability of the method to quantify 
      genetic features at the level of single cells.
FAU - Wang, Xiaozhu
AU  - Wang X
AD  - Department of Chemical and Biomedical Engineering, FAMU-FSU College of 
      Engineering, Florida State University, 2525 Pottsdamer Street, Tallahassee, FL 
      32310, USA.
FAU - Takebayashi, Shin-Ichiro
AU  - Takebayashi S
FAU - Bernardin, Evans
AU  - Bernardin E
FAU - Gilbert, David M
AU  - Gilbert DM
FAU - Chella, Ravindran
AU  - Chella R
FAU - Guan, Jingjiao
AU  - Guan J
LA  - eng
GR  - P01 GM085354/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biomed Microdevices
JT  - Biomedical microdevices
JID - 100887374
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/genetics
MH  - Chromosomes, Mammalian
MH  - DNA/genetics/*isolation & purification
MH  - Embryonic Stem Cells/cytology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mice
MH  - Microfluidic Analytical Techniques/methods
MH  - Microfluidics/*instrumentation/*methods
MH  - Telomere/genetics/metabolism
PMC - PMC3425352
MID - NIHMS397175
EDAT- 2012/01/11 06:00
MHDA- 2012/09/18 06:00
PMCR- 2012/08/22
CRDT- 2012/01/11 06:00
PHST- 2012/01/11 06:00 [entrez]
PHST- 2012/01/11 06:00 [pubmed]
PHST- 2012/09/18 06:00 [medline]
PHST- 2012/08/22 00:00 [pmc-release]
AID - 10.1007/s10544-011-9621-8 [doi]
PST - ppublish
SO  - Biomed Microdevices. 2012 Jun;14(3):443-51. doi: 10.1007/s10544-011-9621-8.