PMID- 22231304
OWN - NLM
STAT- MEDLINE
DCOM- 20120409
LR  - 20220317
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 209
IP  - 1
DP  - 2012 Jan 16
TI  - Inflammation switches the differentiation program of Ly6Chi monocytes from 
      antiinflammatory macrophages to inflammatory dendritic cells in the colon.
PG  - 139-55
LID - 10.1084/jem.20101387 [doi]
AB  - Dendritic cells (DCs) and macrophages (MPs) are important for immunological 
      homeostasis in the colon. We found that F4/80(hi)CX3CR1(hi) (CD11b(+)CD103(-)) 
      cells account for 80% of mouse colonic lamina propria MHC-II(hi) cells. Both 
      CD11c(+) and CD11c(-) cells within this population were identified as MPs based 
      on multiple criteria, including an MP transcriptome revealed by microarray 
      analysis. These MPs constitutively released high levels of IL-10 at least 
      partially in response to the microbiota via an MyD88-independent mechanism. In 
      contrast, cells expressing low to intermediate levels of F4/80 and CX3CR1 were 
      identified as DCs based on phenotypic and functional analysis and comprise three 
      separate CD11c(hi) cell populations: CD103(+)CX3CR1(-)CD11b(-) DCs, 
      CD103(+)CX3CR1(-)CD11b(+) DCs, and CD103(-)CX3CR1(int)CD11b(+) DCs. In 
      noninflammatory conditions, Ly6C(hi) monocytes (MOs) differentiated primarily 
      into CD11c(+) but not CD11c(-) MPs. In contrast, during colitis, Ly6C(hi) MOs 
      massively invaded the colon and differentiated into proinflammatory 
      CD103(-)CX3CR1(int)CD11b(+) DCs, which produced high levels of IL-12, IL-23, 
      iNOS, and TNF. These findings demonstrate the dual capacity of Ly6C(hi) blood MOs 
      to differentiate into either regulatory MPs or inflammatory DCs in the colon and 
      that the balance of these immunologically antagonistic cell types is dictated by 
      microenvironmental conditions.
FAU - Rivollier, Aymeric
AU  - Rivollier A
AD  - Mucosal Immunobiology Section, Laboratory of Molecular Immunology, National 
      Institute of Allergy and Infectious Diseases, National Institutes of Health, 
      Bethesda, MD 20892, USA.
FAU - He, Jianping
AU  - He J
FAU - Kole, Abhisake
AU  - Kole A
FAU - Valatas, Vassilis
AU  - Valatas V
FAU - Kelsall, Brian L
AU  - Kelsall BL
LA  - eng
GR  - T32 GM008169/GM/NIGMS NIH HHS/United States
GR  - ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20120109
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Ly)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Integrin alpha Chains)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Ly-6C antigen, mouse)
RN  - 0 (alpha E integrins)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.7.7.- (Transposases)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Antigens, CD/genetics
MH  - Antigens, Ly/analysis
MH  - Cell Differentiation/*immunology
MH  - Colon/*immunology/metabolism
MH  - Cytokines/biosynthesis/immunology
MH  - Dendritic Cells/cytology/*immunology
MH  - Inflammation/*immunology/metabolism
MH  - Inflammation Mediators/immunology/metabolism
MH  - Integrin alpha Chains/deficiency/genetics
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-10/biosynthesis/immunology
MH  - Lipopolysaccharides/immunology
MH  - Macrophages/cytology/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Monocytes/*cytology
MH  - Mucous Membrane/immunology/metabolism
MH  - T-Lymphocytes/immunology/metabolism
MH  - Transposases/genetics
PMC - PMC3260867
EDAT- 2012/01/11 06:00
MHDA- 2012/04/10 06:00
PMCR- 2012/07/16
CRDT- 2012/01/11 06:00
PHST- 2012/01/11 06:00 [entrez]
PHST- 2012/01/11 06:00 [pubmed]
PHST- 2012/04/10 06:00 [medline]
PHST- 2012/07/16 00:00 [pmc-release]
AID - jem.20101387 [pii]
AID - 20101387 [pii]
AID - 10.1084/jem.20101387 [doi]
PST - ppublish
SO  - J Exp Med. 2012 Jan 16;209(1):139-55. doi: 10.1084/jem.20101387. Epub 2012 Jan 9.