PMID- 22232624
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20211021
IS  - 2092-7258 (Electronic)
IS  - 1738-1061 (Print)
IS  - 1738-1061 (Linking)
VI  - 54
IP  - 10
DP  - 2011 Oct
TI  - Identification of major rice allergen and their clinical significance in 
      children.
PG  - 414-21
LID - 10.3345/kjp.2011.54.10.414 [doi]
AB  - PURPOSE: Recently, an increase in the number of patients sensitized to rice 
      allergen with or without clinical symptoms has been reported. This study was 
      designed to determine the major allergens in rice and their clinical 
      significance. METHODS: Twenty-four children (15 boys and 9 girls; mean age, 16.3 
      months) with allergic disease, who were sensitized to rice antigen (by UniCAP) in 
      the Pediatric Allergy Respiratory Center at Soonchunhyang University Hospital, 
      were enrolled in this study. The allergenicity of various types of rice (raw, 
      cooked, and heat-treated, simulated gastric fluid [SGF], and simulated intestinal 
      fluid [SIF]) was investigated using sodium dodecyl sulfate-polyacrylamide gel 
      electrophoresis (SDS-PAGE) and immunoglobulin E (IgE) immunoblots. The patients' 
      medical records, including laboratory data and allergy symptoms after ingestion 
      of rice were reviewed. RESULTS: Patients were sensitized to an average of 13.5 
      food antigens and their mean total IgE was 6,888.7 kU/L. In SDS-PAGE, more than 
      16 protein bands were observed in the raw rice, whereas only 14-16 kDa and 31-35 
      kDa protein bands were observed in cooked rice. The common SDS-PAGE protein bands 
      observed in SGF-, SIF-, and heat-treated rice were 9, 14, and 31 kDa. In a 
      heated-rice IgE immunoblot, protein bands of 9, 14, and 31-33 kDa were found in 
      27.8%, 38.9%, and 38.9% of all sera, respectively, and in 50%, 50%, and 75%, of 
      ser a from the 4 symptomatic patients, respectively. CONCLUSION: The 9-, 14-, and 
      31-kDa protein bands appeared to be the major allergens responsible for rice 
      allergy symptoms.
FAU - Jeon, You Hoon
AU  - Jeon YH
AD  - Department of Pediatrics, Soonchunhyang University School of Medicine, Seoul, 
      Korea.
FAU - Oh, Se Jo
AU  - Oh SJ
FAU - Yang, Hyeon Jong
AU  - Yang HJ
FAU - Lee, Soo Young
AU  - Lee SY
FAU - Pyun, Bok Yang
AU  - Pyun BY
LA  - eng
PT  - Journal Article
DEP - 20111031
PL  - Korea (South)
TA  - Korean J Pediatr
JT  - Korean journal of pediatrics
JID - 101215374
PMC - PMC3250595
OTO - NOTNLM
OT  - Child
OT  - Rice allergen
OT  - Sensitization
EDAT- 2012/01/11 06:00
MHDA- 2012/01/11 06:01
PMCR- 2011/10/01
CRDT- 2012/01/11 06:00
PHST- 2011/01/10 00:00 [received]
PHST- 2011/04/06 00:00 [revised]
PHST- 2011/09/23 00:00 [accepted]
PHST- 2012/01/11 06:00 [entrez]
PHST- 2012/01/11 06:00 [pubmed]
PHST- 2012/01/11 06:01 [medline]
PHST- 2011/10/01 00:00 [pmc-release]
AID - 10.3345/kjp.2011.54.10.414 [doi]
PST - ppublish
SO  - Korean J Pediatr. 2011 Oct;54(10):414-21. doi: 10.3345/kjp.2011.54.10.414. Epub 
      2011 Oct 31.