PMID- 22233079
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20120906
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 9
DP  - 2011 Sep
TI  - [Development and evaluation of a novel three-dimensional adjustable confocal 
      laser-induced fluorescence detector].
PG  - 896-900
AB  - Laser-induced fluorescence detector (LIFD) is one of the most sensitive detectors 
      in analytical chemical files. Confocal optical configuration is widely used in 
      LIFDs. Two effective approaches used to achieve the best signal to noise ratio 
      (S/N) are increasing the confocal precision and minimizing the background noise. 
      A novel three-dimensional adjustable confocal LIFD was developed, using a new 
      three-dimensional adjustable supporter of reflector and modularized optical 
      system. A detection limit (S/N = 3) of 1 x 10(-12) mol/L and a linear dynamic 
      range of 3 orders of magnitude were obtained using fluorescein isothiocyanate 
      (FITC) standard as the test sample. The noise level and drift levels were 8.0 x 
      10(-3) mV and 1.4 x 10(-3) mV/h, respectively, which were almost 10 times lower 
      than before. And the stability of the LIFD was evaluated by five replicate 
      injections of 5 x 10(-9) mol/L FITC, and the relative standard deviations (RSDs) 
      of peak height and peak area were 0.38% and 0.41%, respectively. Further more, 
      three biogenic amines, which were derivatized by FITC, were separated by high 
      performance liquid chromatography (HPLC) and then detected by the novel LIFD. And 
      the detection limits (S/N = 3) ranged 0.01 to 0.02 nmol/L, which were better than 
      other methods. Therefore, the LIFD is highly sensitive, as well as shows a real 
      low noise level and good reproducibility.
FAU - Liu, Fan
AU  - Liu F
AD  - Shanghai Key Laboratory of Functional Materials Chemistry, East China University 
      of Science and Technology, Shanghai 200237, China.
FAU - Yu, Shuxin
AU  - Yu S
FAU - Tang, Tao
AU  - Tang T
FAU - Sun, Yuanshe
AU  - Sun Y
FAU - Zhang, Weibing
AU  - Zhang W
FAU - Li, Tong
AU  - Li T
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
EDAT- 2012/01/12 06:00
MHDA- 2012/01/12 06:01
CRDT- 2012/01/12 06:00
PHST- 2012/01/12 06:00 [entrez]
PHST- 2012/01/12 06:00 [pubmed]
PHST- 2012/01/12 06:01 [medline]
PST - ppublish
SO  - Se Pu. 2011 Sep;29(9):896-900.