PMID- 22233085
OWN - NLM
STAT- MEDLINE
DCOM- 20120906
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 9
DP  - 2011 Sep
TI  - [Accurate determination of chloramphenicol in pork by isotope dilution liquid 
      chromatography-electrospray ionization tandem mass spectrometry].
PG  - 927-31
AB  - An accurate method was described for the determination of chloramphenicol (CAP) 
      in pork. The analyte was quantified by isotope dilution liquid 
      chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) 
      operating in negative ion multiple reaction monitoring (MRM) mode. The sample was 
      treated by liquid-liquid extraction with ethyl acetate followed by a clean-up 
      step on an HLB solid-phase extraction cartridge. Chloramphenicol-D5 (CAP-D5) was 
      added as an isotope internal standard. Under the optimized conditions, the 
      calibration curve showed a good linearity in ranging from 0.01 to 0.5 ng/g CAP. 
      The correlation coefficient r2 was 0.9998. The limit of detection and the limit 
      of quantification were 0.004 and 0.02 ng/g, respectively. The average recoveries 
      of CAP at 0.02 ng/g and 1.0 ng/g spiked levels ranged from 95.2%-109.1%, 
      99.7%-102.5%, respectively. The relative standard deviations (RSDs) of intra-day 
      and inter-day were less than 2%. Meanwhile, matrix effect factors k of CAP and 
      CAP-D5 were determined under different mobile phases and the sample solvent 
      composition, the k was in the range of 0.950-1.015. The method can be used as a 
      confirmed method for the determination of CAP in pork.
FAU - Yang, Zong
AU  - Yang Z
AD  - National Institute of Metrology, Beijing 100013, China.
FAU - Li, Xiuqin
AU  - Li X
FAU - Wang, Juan
AU  - Wang J
FAU - Zhang, Qinghe
AU  - Zhang Q
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Isotopes)
RN  - 66974FR9Q1 (Chloramphenicol)
SB  - IM
MH  - Animals
MH  - Chloramphenicol/*analysis
MH  - Chromatography, Liquid/*methods
MH  - Drug Residues/*analysis
MH  - Food Analysis/methods
MH  - Food Contamination/analysis
MH  - Isotopes
MH  - Meat/*analysis
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Swine
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2012/01/12 06:00
MHDA- 2012/09/07 06:00
CRDT- 2012/01/12 06:00
PHST- 2012/01/12 06:00 [entrez]
PHST- 2012/01/12 06:00 [pubmed]
PHST- 2012/09/07 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Sep;29(9):927-31.