PMID- 22233687
OWN - NLM
STAT- MEDLINE
DCOM- 20120508
LR  - 20120213
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 32
IP  - 4
DP  - 2012 Apr
TI  - Diagnostic capacity for viral haemorrhagic septicaemia virus (VHSV) infection in 
      rainbow trout (Oncorhynchus mykiss) is greatly increased by combining viral 
      isolation with specific antibody detection.
PG  - 593-7
LID - 10.1016/j.fsi.2011.12.007 [doi]
AB  - Detection of disease specific antibodies in farmed rainbow trout (Oncorhynchus 
      mykiss) has been proposed as an alternative or supplement to the currently 
      approved procedures for diagnosis and surveillance in this species. In samples 
      from natural outbreaks of the disease viral haemorrhagic septicaemia (VHS) at two 
      freshwater farms in southern Denmark serologic testing was used to broaden the 
      diagnostic window from outbreak to diagnosis in the laboratory as compared to 
      traditional procedures of isolation and identification of the virus. The 
      serologic assay clearly increased the chance of detecting present or previous 
      infections where the pathogen could not be isolated by standard methods 
      (indicating older infections where the virus had been cleared). Our data allowed 
      us to monitor the levels of neutralising antibodies in relation to the presence 
      of the virus in fish experiencing two different types of outbreaks at two 
      different farms. By sequence analysis of the viral glycoprotein from selected 
      isolates we found no evidence for escape mutants having developed in the fish 
      showing high titres of neutralising antibodies.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Schyth, B D
AU  - Schyth BD
AD  - National Veterinary Institute, Technical University of Denmark, Hangovej 2, 8200 
      Arhus N., Denmark. bdsc@vet.dtu.dk
FAU - Ariel, E
AU  - Ariel E
FAU - Korsholm, H
AU  - Korsholm H
FAU - Olesen, N J
AU  - Olesen NJ
LA  - eng
PT  - Journal Article
DEP - 20111222
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing
MH  - Antibodies, Viral/*blood
MH  - Antibody Specificity
MH  - Hemorrhagic Septicemia, Viral/blood/*diagnosis/virology
MH  - Novirhabdovirus/*isolation & purification
MH  - *Oncorhynchus mykiss
MH  - Reproducibility of Results
MH  - Serologic Tests
EDAT- 2012/01/12 06:00
MHDA- 2012/05/09 06:00
CRDT- 2012/01/12 06:00
PHST- 2011/09/30 00:00 [received]
PHST- 2011/11/28 00:00 [revised]
PHST- 2011/12/13 00:00 [accepted]
PHST- 2012/01/12 06:00 [entrez]
PHST- 2012/01/12 06:00 [pubmed]
PHST- 2012/05/09 06:00 [medline]
AID - S1050-4648(11)00460-8 [pii]
AID - 10.1016/j.fsi.2011.12.007 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2012 Apr;32(4):593-7. doi: 10.1016/j.fsi.2011.12.007. 
      Epub 2011 Dec 22.