PMID- 22234851
OWN - NLM
STAT- MEDLINE
DCOM- 20120921
LR  - 20240109
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Linking)
VI  - 19
IP  - 6
DP  - 2012 Jul
TI  - Phytoremediation of 2,4-dichlorophenol using wild type and transgenic tobacco 
      plants.
PG  - 2202-11
LID - 10.1007/s11356-011-0724-9 [doi]
AB  - INTRODUCTION: Transgenic plant strategies based on peroxidase expression or 
      overexpression would be useful for phenolic compound removal since these enzymes 
      play an important role in phenolic polymerizing reactions. MATERIAL AND METHODS: 
      Thus, double transgenic (DT) plants for basic peroxidases were obtained and 
      characterized in order to compare the tolerance and efficiency for 
      2,4-dichlorophenol (2,4-DCP) removal with WT and simple transgenic plants 
      expressing TPX1 or TPX2 gene. Several DT plants showed the expression of both 
      transgenes and proteins, as well as increased peroxidase activity. RESULTS: DT 
      lines showed higher tolerance to 2,4-DCP at early stage of development since 
      their germination index was higher than that of WT seedlings exposed to 25 mg/L 
      of the pollutant. High 2,4-DCP removal efficiencies were found for WT tobacco 
      plants. TPX1 transgenic plants and DT (line d) reached slightly higher removal 
      efficiencies for 10 mg/L of 2,4-DCP than WT plants, while DT plants (line A) 
      showed the highest removal efficiencies (98%). These plants showed an increase of 
      21% and 14% in 2,4-DCP removal efficiency for solutions containing 10 and 25 mg/L 
      2,4-DCP, respectively, compared with WT plants. In addition, an almost complete 
      toxicity reduction of postremoval solutions using WT and DT plants was obtained 
      through AMPHITOX test, which indicates that the 2,4-DCP degradation products 
      would be similar for both plants. CONCLUSION: These results are relevant in the 
      field of phytoremediation application and, moreover, they highlight the safety of 
      using DT tobacco plants because nontoxic products were formed after an efficient 
      2,4-DCP removal.
FAU - Talano, Melina A
AU  - Talano MA
AD  - Departamento de Biologia Molecular, FCEFQyN, Universidad Nacional de Rio Cuarto, 
      Ruta Nacional 36 Km 601, CP 5800, Rio Cuarto, Cordoba, Argentina. 
      melinatalano@hotmail.com
FAU - Busso, Debora C
AU  - Busso DC
FAU - Paisio, Cintia E
AU  - Paisio CE
FAU - Gonzalez, Paola S
AU  - Gonzalez PS
FAU - Purro, Silvia A
AU  - Purro SA
FAU - Medina, Maria I
AU  - Medina MI
FAU - Agostini, Elizabeth
AU  - Agostini E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120111
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (Chlorophenols)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Blotting, Northern
MH  - Chlorophenols/*metabolism
MH  - Germination/drug effects
MH  - Peroxidase/genetics/metabolism
MH  - Plants, Genetically Modified/drug effects/growth & development/*metabolism
MH  - Nicotiana/drug effects/genetics/growth & development/*metabolism
EDAT- 2012/01/12 06:00
MHDA- 2012/09/22 06:00
CRDT- 2012/01/12 06:00
PHST- 2011/04/30 00:00 [received]
PHST- 2011/12/27 00:00 [accepted]
PHST- 2012/01/12 06:00 [entrez]
PHST- 2012/01/12 06:00 [pubmed]
PHST- 2012/09/22 06:00 [medline]
AID - 10.1007/s11356-011-0724-9 [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2012 Jul;19(6):2202-11. doi: 
      10.1007/s11356-011-0724-9. Epub 2012 Jan 11.