PMID- 22237534
OWN - NLM
STAT- MEDLINE
DCOM- 20120502
LR  - 20120112
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 840
DP  - 2012
TI  - Case study: targeted high-throughput sequencing of mitochondrial genomes from 
      extinct cave bears via direct multiplex PCR sequencing (DMPS).
PG  - 171-6
LID - 10.1007/978-1-61779-516-9_20 [doi]
AB  - Here I describe the use of a recently developed technique for targeted 
      high-throughput sequencing of highly degraded DNA by direct multiplex PCR 
      sequencing (DMPS) that was used to amplify 31 near-complete mitochondrial genomes 
      of the extinct cave bear (Ursus spelaeus). DMPS couples multiplex PCR with the 
      generation of barcoded sequencing libraries to be sequenced in parallel on a 
      high-throughput sequencing platform. DMPS makes it possible to generate large 
      amounts of targeted DNA sequence data simultaneously from multiple degraded 
      samples such as fossil remains. In this chapter, I describe an experiment that 
      uses DMPS with different primer sets and on both modern and ancient DNA 
      templates.
FAU - Stiller, Mathias
AU  - Stiller M
AD  - Department of Biology, The Pennsylvania State University, 320 Mueller Laboratory, 
      University Park, PA 16802, USA. mus52@psu.edu
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA, Mitochondrial)
SB  - IM
MH  - Animals
MH  - DNA, Mitochondrial/*genetics
MH  - *Extinction, Biological
MH  - Fossils
MH  - *Genome, Mitochondrial
MH  - High-Throughput Nucleotide Sequencing/*methods
MH  - Multiplex Polymerase Chain Reaction/*methods
MH  - Ursidae/*genetics
EDAT- 2012/01/13 06:00
MHDA- 2012/05/04 06:00
CRDT- 2012/01/13 06:00
PHST- 2012/01/13 06:00 [entrez]
PHST- 2012/01/13 06:00 [pubmed]
PHST- 2012/05/04 06:00 [medline]
AID - 10.1007/978-1-61779-516-9_20 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2012;840:171-6. doi: 10.1007/978-1-61779-516-9_20.