PMID- 22238454
OWN - NLM
STAT- MEDLINE
DCOM- 20120326
LR  - 20201222
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 188
IP  - 4
DP  - 2012 Feb 15
TI  - Conventional dendritic cells require IRAP-Rab14 endosomes for efficient 
      cross-presentation.
PG  - 1840-6
LID - 10.4049/jimmunol.1101504 [doi]
AB  - Dendritic cells (DCs) use cellular pathways collectively referred to as 
      cross-presentation to stimulate CD8(+) T cells with peptide Ags derived from 
      internalized, exogenous Ags. We have recently reported that DCs rely on 
      aminoterminal trimming of cross-presented peptides by insulin-responsive 
      aminopeptidase (IRAP), an enzyme localized in a regulated endosomal storage 
      compartment. Considering a report contending that this role is limited to 
      inflammatory DCs (Segura et al. 2009. Proc. Natl. Acad. Sci. USA 106: 
      20377-20381), in this study, we examined the role of IRAP in steady-state DC 
      subpopulations. Steady-state conventional DCs (cDCs) and plasmacytoid DCs 
      expressed similar amounts of IRAP. IRAP colocalized with the endosomal markers 
      Rab14 and syntaxin 6, both known to be associated with regulated endosomal 
      storage compartments, in CD8(+) and CD8(-) cDCs-however, to a greater extent in 
      the former population. Likewise, IRAP recruitment to phagosomes was significantly 
      stronger in CD8(+) DCs. IRAP deficiency compromised cross-presentation of soluble 
      and particulate Ag by both CD8(+) and CD8(-) cDCs, again with a stronger effect 
      in the former population. Thus, the requirement of IRAP in cross-presentation 
      extends to steady-state cDCs. Moreover, these data suggest that increased 
      recruitment of an IRAP(+)/Rab14(+) compartment to Ag-containing vesicles 
      contributes to the superior cross-presentation efficacy of CD8(+) cDCs.
FAU - Weimershaus, Mirjana
AU  - Weimershaus M
AD  - INSERM, Unite 1013, Paris 75015, France.
FAU - Maschalidi, Sophia
AU  - Maschalidi S
FAU - Sepulveda, Fernando
AU  - Sepulveda F
FAU - Manoury, Benedicte
AU  - Manoury B
FAU - van Endert, Peter
AU  - van Endert P
FAU - Saveanu, Loredana
AU  - Saveanu L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120111
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Qa-SNARE Proteins)
RN  - EC 3.4.11.3 (Cystinyl Aminopeptidase)
RN  - EC 3.4.11.3 (leucyl-cystinyl aminopeptidase)
RN  - EC 3.6.1.- (Rab14 protein, mouse)
RN  - EC 3.6.5.2 (rab GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - CD8-Positive T-Lymphocytes/immunology/metabolism
MH  - Cells, Cultured
MH  - *Cross-Priming
MH  - Cystinyl Aminopeptidase/biosynthesis/*metabolism
MH  - Dendritic Cells/*immunology/metabolism
MH  - Endosomes/*immunology/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Phagosomes/immunology/metabolism
MH  - Qa-SNARE Proteins/metabolism
MH  - rab GTP-Binding Proteins/*metabolism
EDAT- 2012/01/13 06:00
MHDA- 2012/03/27 06:00
CRDT- 2012/01/13 06:00
PHST- 2012/01/13 06:00 [entrez]
PHST- 2012/01/13 06:00 [pubmed]
PHST- 2012/03/27 06:00 [medline]
AID - jimmunol.1101504 [pii]
AID - 10.4049/jimmunol.1101504 [doi]
PST - ppublish
SO  - J Immunol. 2012 Feb 15;188(4):1840-6. doi: 10.4049/jimmunol.1101504. Epub 2012 
      Jan 11.