PMID- 22238752
OWN - NLM
STAT- MEDLINE
DCOM- 20120525
LR  - 20190813
IS  - 1573-8221 (Electronic)
IS  - 0007-4888 (Linking)
VI  - 151
IP  - 2
DP  - 2011 Jun
TI  - Age-associated reduction of the count and functional activity of stromal 
      precursor cells can be caused by both true reduction (exhaustion) of cell pool 
      and regulatory effects of the organism.
PG  - 210-4
AB  - The study was carried out on CBA mice using the method of heterotopic 
      transplantation. A fragment of the femoral bone marrow (1/2) or spleen (1/5 of 
      the organ) was transplanted under the renal capsule of a recipient. The following 
      donor-recipient cross-transplantation variants were studied: young-young (Y-Y), 
      young-old (Y-O), old-old (O-O), and old-young (O-Y). Cell suspensions were 
      prepared from 2-month transplants inoculated in monolayer cultures and the 
      cloning efficiency (ECF-F) of stromal precursor cells (CFC-F) was evaluated. The 
      bone marrow transplant ECF-F and the count of CFC-F in the O-O group were 8-fold 
      lower than in the Y-Y group. In the O-Y group, ECF-F was 3-fold higher than in 
      the O-O group, but by 2.5 times lower than in the Y-Y group. ECF-F in Y-O group 
      was 2-fold lower than in Y-Y group. The ECF-F and CFC-F count in spleen 
      transplants in the O-O group were 4- and 6-fold lower, respectively, than in Y-Y 
      group. However, in O-Y group ECF-F was 7-fold higher than in O-O group and higher 
      than even in Y-Y group. The weight of induced ectopic bone tissue after 
      transplantation of the osteoinductor (fragments of the allogenic urinary bladder 
      mucosa) was 2-fold lower in the O-O vs. Y-Y group. However, comparison of the 
      ectopic bone tissue weights in different experimental groups showed that 
      osteoinductor activity of the bladder epithelium did not decrease, but increased 
      3-fold with age (O-Y:Y-Y). A 5-fold reduction of this proportion in groups where 
      the osteoinductor was transplanted from old donors to old and young recipients 
      (O-Y:O-O) could be attributed to age-specific reduction of the count of inducible 
      osteogenic precursor cells (IOPC). The data in general suggest that age-specific 
      reduction of the stromal precursor count and functional activity could be caused 
      by the true reduction (exhaustion) of cell pool (bone marrow CFC-F; presumably, 
      IOPC) and by the regulatory effects of the organism (bone marrow and splenic 
      CFC-F, IOPC). These data seem to be significant for understanding of the role of 
      osteogenic stromal precursor cells in the development of age-associated bone 
      tissue defects, for example, senile osteoporosis.
FAU - Gorskaya, Yu F
AU  - Gorskaya YF
AD  - Laboratory of Immunity Regulation, N. F. Gamaleya Institute of Epidemiology and 
      Microbiology, Russian Academy of Medical Sciences, Moscow, Russia. 
      uliya.gorskaya@nearmedic.ru
FAU - Danilova, T A
AU  - Danilova TA
FAU - Nesterenko, V G
AU  - Nesterenko VG
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Bull Exp Biol Med
JT  - Bulletin of experimental biology and medicine
JID - 0372557
SB  - IM
MH  - Age Factors
MH  - Aging
MH  - Animals
MH  - Bone Marrow Transplantation
MH  - Cell Count
MH  - Cell Culture Techniques
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Guinea Pigs
MH  - Male
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Osteogenesis
MH  - Spleen/cytology/transplantation
MH  - Stem Cells/*cytology
MH  - Stromal Cells/*cytology
MH  - Transplantation, Heterotopic
MH  - Urinary Bladder/cytology/transplantation
EDAT- 2012/01/13 06:00
MHDA- 2012/05/26 06:00
CRDT- 2012/01/13 06:00
PHST- 2012/01/13 06:00 [entrez]
PHST- 2012/01/13 06:00 [pubmed]
PHST- 2012/05/26 06:00 [medline]
AID - 10.1007/s10517-011-1291-2 [doi]
PST - ppublish
SO  - Bull Exp Biol Med. 2011 Jun;151(2):210-4. doi: 10.1007/s10517-011-1291-2.