PMID- 22239468
OWN - NLM
STAT- MEDLINE
DCOM- 20130220
LR  - 20120924
IS  - 1440-1789 (Electronic)
IS  - 0919-6544 (Linking)
VI  - 32
IP  - 5
DP  - 2012 Oct
TI  - Transplanted bone marrow stromal cells protect neurovascular units and ameliorate 
      brain damage in stroke-prone spontaneously hypertensive rats.
PG  - 522-33
LID - 10.1111/j.1440-1789.2011.01291.x [doi]
AB  - This study was aimed to assess whether bone marrow stromal cells (BMSC) could 
      ameliorate brain damage when transplanted into the brain of stroke-prone 
      spontaneously hypertensive rats (SHR-SP). The BMSC or vehicle was 
      stereotactically engrafted into the striatum of male SHR-SP at 8 weeks of age. 
      Daily loading with 0.5% NaCl-containing water was started from 9 weeks. MRIs and 
      histological analysis were performed at 11 and 12 weeks, respectively. 
      Wistar-Kyoto rats were employed as the control. As a result, T2-weighted images 
      demonstrated neither cerebral infarct nor intracerebral hemorrhage, but 
      identified abnormal dilatation of the lateral ventricles in SHR-SP. HE staining 
      demonstrated selective neuronal injury in their neocortices. Double fluorescence 
      immunohistochemistry revealed that they had a decreased density of the collagen 
      IV-positive microvessels and a decreased number of the microvessels with normal 
      integrity between basement membrane and astrocyte end-feet. BMSC transplantation 
      significantly ameliorated the ventricular dilatation and the breakdown of 
      neurovascular integrity. These findings strongly suggest that long-lasting 
      hypertension may primarily damage neurovascular integrity and neurons, leading to 
      tissue atrophy and ventricular dilatation prior to the occurrence of cerebral 
      stroke. The BMSC may ameliorate these damaging processes when directly 
      transplanted into the brain, opening the possibility of prophylactic medicine to 
      prevent microvascular and parenchymal-damaging processes in hypertensive patients 
      at higher risk for cerebral stroke.
CI  - (c) 2012 Japanese Society of Neuropathology.
FAU - Ito, Masaki
AU  - Ito M
AD  - Department of Neurosurgery, Hokkaido University Graduate School of Medicine, 
      Sapporo, Japan.
FAU - Kuroda, Satoshi
AU  - Kuroda S
FAU - Sugiyama, Taku
AU  - Sugiyama T
FAU - Maruichi, Katsuhiko
AU  - Maruichi K
FAU - Kawabori, Masahito
AU  - Kawabori M
FAU - Nakayama, Naoki
AU  - Nakayama N
FAU - Houkin, Kiyohiro
AU  - Houkin K
FAU - Iwasaki, Yoshinobu
AU  - Iwasaki Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120112
PL  - Australia
TA  - Neuropathology
JT  - Neuropathology : official journal of the Japanese Society of Neuropathology
JID - 9606526
SB  - IM
MH  - Animals
MH  - Blood Pressure
MH  - Blood Vessels/*pathology
MH  - Bone Marrow Transplantation/*methods
MH  - Brain/*pathology
MH  - Cerebral Ventricles/pathology
MH  - Fluorescent Antibody Technique, Indirect
MH  - Immunohistochemistry
MH  - Magnetic Resonance Imaging
MH  - Male
MH  - Myocytes, Smooth Muscle/*pathology
MH  - Neostriatum/pathology
MH  - Neurons/*pathology
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
MH  - Stereotaxic Techniques
MH  - Stroke/*pathology/*therapy
MH  - Stromal Cells/*transplantation
EDAT- 2012/01/14 06:00
MHDA- 2013/02/21 06:00
CRDT- 2012/01/14 06:00
PHST- 2012/01/14 06:00 [entrez]
PHST- 2012/01/14 06:00 [pubmed]
PHST- 2013/02/21 06:00 [medline]
AID - 10.1111/j.1440-1789.2011.01291.x [doi]
PST - ppublish
SO  - Neuropathology. 2012 Oct;32(5):522-33. doi: 10.1111/j.1440-1789.2011.01291.x. 
      Epub 2012 Jan 12.