PMID- 22242353
OWN - NLM
STAT- MEDLINE
DCOM- 20120412
LR  - 20181201
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 25
IP  - 12
DP  - 2011 Dec
TI  - [Experimental study on ectopic bone formation of chitosan/phosphonic chitosan 
      sponge combined with human umbilical cord mesenchymal stem cells].
PG  - 1493-8
AB  - OBJECTIVE: To investigate the ectopic bone formation of the chitosan/phosphonic 
      chitosan sponge combined with human umbilical cord mesenchymal stem cells 
      (hUCMSCs) in vitro. METHODS: Phosphorous groups were introduced in chitosan 
      molecules to prepare the phosphonic chitosan; 2% chitosan and phosphonic chitosan 
      solutions were mixed at a volume ratio of 1 : 1 and freeze-dried to build the 
      complex sponge, and then was put in the simulated body fluid for biomimetic 
      mineralization in situ. The hUCMSCs were isolated by enzyme digestion method from 
      human umbilical cord and were cultured. The chitosan/phosphonic chitosan sponge 
      was cultured with hUCMSCs at passage 3, and the cell-scaffold composite was 
      cultured in osteogenic medium. The growth and adhesion of the cells on the 
      scaffolds were observed by light microscope and scanning electron microscope 
      (SEM) at 1 and 2 weeks after culturing, respectively. The cell proliferation was 
      detected by MTT assay at 1, 2, 3, 4, 5, and 6 days, respectively. Bilateral back 
      muscles defects were created on 40 New Zealand rabbits (3-4 months old, weighing 
      2.1-3.2 kg, male or female), which were divided into groups A, B, and C. In group 
      A, cell-scaffold composites were implanted into 40 right defects; in group B, the 
      complex sponge was implanted into 20 left defects; and in group C, none was 
      implanted into other 20 left defects. The gross and histological observations 
      were made at 4 weeks RESULTS: The analysis results of phosphonic chitosan showed 
      that the phosphorylation occurred mainly in the hydroxyl, and the proton type and 
      chemical shifts intensity were conform to its chemical structure. The SEM results 
      showed that the pores of the chitosan/phosphonic chitosan sponge were 
      homogeneous, and the wall of the pore was thinner; the coating of calcium and 
      phosphorus could be observed on the surface of the pore wall after mineralized 
      with crystal particles; the cells grew well on the surface of the 
      chitosan/phosphonic chitosan sponge. The MTT assay showed that the 
      chitosan/phosphonic chitosan sponge could not inhibit the proliferation of 
      hUCMSCs. The gross observation showed that the size and shape of the 
      cell-scaffold composite remained intact and texture was toughened in group A, the 
      size of the complex sponge gradually reduced in group B, and the muscle defects 
      wound healed with a little scar tissue in group C. The histological observation 
      showed that part of the scaffold was absorbed and new blood vessels and new bone 
      trabeculae formed in group A, the circular cavity and residual chitosan scaffolds 
      were observed in group B, and the wound almost healed with a small amount of 
      lymphocytes in group C. CONCLUSION: The chitosan/phosphonic chitosan sponge has 
      good biocompatibility, the tissue engineered bone by combining the hUCMSCs with 
      chitosan/phosphonic chitosan sponge has the potential of the ectopic bone 
      formation in rabbit.
FAU - Sun, Guodong
AU  - Sun G
AD  - Department of Orthopaedics, First Affiliated Hospital of Jinan University, 
      Guangzhou Guangdong 510630, PR China.
FAU - Li, Zhizhong
AU  - Li Z
FAU - Jiao, Yanpeng
AU  - Jiao Y
FAU - Zhou, Changren
AU  - Zhou C
FAU - Jiao, Genlong
AU  - Jiao G
FAU - Lin, Yongxin
AU  - Lin Y
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Biocompatible Materials)
RN  - 0 (Organophosphorus Compounds)
RN  - 115-78-6 (phosphon)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Animals
MH  - *Biocompatible Materials
MH  - Bone Regeneration
MH  - Chitosan
MH  - *Cord Blood Stem Cell Transplantation
MH  - Female
MH  - Humans
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology
MH  - Organophosphorus Compounds
MH  - *Osteogenesis
MH  - Rabbits
EDAT- 2012/01/17 06:00
MHDA- 2012/04/13 06:00
CRDT- 2012/01/17 06:00
PHST- 2012/01/17 06:00 [entrez]
PHST- 2012/01/17 06:00 [pubmed]
PHST- 2012/04/13 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2011 Dec;25(12):1493-8.