PMID- 22242457
OWN - NLM
STAT- MEDLINE
DCOM- 20130603
LR  - 20131121
IS  - 0513-4870 (Print)
IS  - 0513-4870 (Linking)
VI  - 46
IP  - 10
DP  - 2011 Oct
TI  - [Simultaneous determination of seven flavonoids in Nervilia fordii with HPLC].
PG  - 1237-40
AB  - The study is to develop an HPLC method for simultaneous determination of 
      rhamnazin (1), rhamnocitrin (2), rhamnetin (3), 
      rhamnazin-3-O-beta-D-glucopyranoside (4), 
      rhamnazin-3-O-beta-D-xylopyranosyl-(1-->4)-beta-D-glucopyranoside (5), 
      rhamnazin-3-O-beta-D-glucopyranosyl-(1-->4)-beta-D-glucopyranoside (6), and 
      rhamnocitrin-3-O-beta-D-glucopyranosyl-(1-->4)-beta-D-glucopyranoside (7) in 
      Nervilia fordii. The separation was performed on a Kromasil C18 column (250 mm x 
      4.6 mm, 5 microm) with 0.4% phosphoric acid-acetonitrile as the mobile phase in a 
      gradient elution at a flow rate of 1.0 mL x min(-1). The detect wavelength was 
      set at 256 nm, and the column temperature was set at 40 degrees C. There were 
      good linear relationships between the logarithm values of concentrations and 
      those of the peak areas of seven flavonoids (1-7) in the range of 0.55-70.00 
      microg x mL(-1) (r = 0.9997), 0.86-110.00 microg x mL(-1) (r = 0.9997), 
      0.39-50.00 microg x mL(-1) (r = 0.999 7), 0.55-70.00 microg x mL(-1) (r = 0.999 
      5), 1.33-170.00 microg x mL(-1) (r = 0.9998), 1.33-170.00 microg x mL(-1) (r = 
      0.9998), 0.16-20.00 microg x mL(-1) (r = 0.9995), respectively. The recoveries of 
      the seven flavonoids were between 97.19%-99.45%, the relative standard deviations 
      (RSDs) were between 0.91%-2.69%. The established method is rapid, accurate with 
      high repeatability, which could provide scientific evidence for the quality 
      control of Nervilia fordii.
FAU - Zhang, Li
AU  - Zhang L
AD  - School of Chinese Materia Medica, Guangzhou University of Traditional Chinese 
      Medicine, Guangzhou 510006, China.
FAU - Zhu, Chen-chen
AU  - Zhu CC
FAU - Zhao, Zhong-xiang
AU  - Zhao ZX
FAU - Lin, Chao-zhan
AU  - Lin CZ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yao Xue Xue Bao
JT  - Yao xue xue bao = Acta pharmaceutica Sinica
JID - 21710340R
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavonoids)
RN  - 0 (Kaempferols)
RN  - 0 (kaempferol-7-methyl ether)
RN  - 71803L5F4S (rhamnetin)
RN  - 9IKM0I5T1E (Quercetin)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Drugs, Chinese Herbal/analysis
MH  - Flavonoids/*analysis
MH  - Kaempferols/analysis
MH  - Orchidaceae/*chemistry
MH  - Plant Leaves/chemistry
MH  - Plants, Medicinal/chemistry
MH  - Quality Control
MH  - Quercetin/analogs & derivatives/analysis
EDAT- 2012/01/17 06:00
MHDA- 2013/06/05 06:00
CRDT- 2012/01/17 06:00
PHST- 2012/01/17 06:00 [entrez]
PHST- 2012/01/17 06:00 [pubmed]
PHST- 2013/06/05 06:00 [medline]
PST - ppublish
SO  - Yao Xue Xue Bao. 2011 Oct;46(10):1237-40.