PMID- 22244632
OWN - NLM
STAT- MEDLINE
DCOM- 20120621
LR  - 20161125
IS  - 1878-3554 (Electronic)
IS  - 0099-2399 (Linking)
VI  - 38
IP  - 2
DP  - 2012 Feb
TI  - Expressional alterations of fibrillin-1 during wound healing of human dental 
      pulp.
PG  - 177-84
LID - 10.1016/j.joen.2011.09.016 [doi]
AB  - INTRODUCTION: The degradation of fibrillins, the major constituents of 
      microfibrils, is known to facilitate the release of active transforming growth 
      factor-beta (TGF-beta), a signaling molecule contributing to mineralized tissue barrier 
      formation in exposed dental pulps. To examine the involvement of fibrillins in 
      the barrier formation, we examined the temporospatial expression of (1) genes and 
      proteins of fibrillins and (2) factors possibly associated with fibrillin 
      degradation and cytodifferentiation in exposed human pulps. Human pulp slice 
      cultures were also examined for the role of fibrillins in mineralization. 
      METHODS: Clinically healthy pulps were mechanically exposed and capped with 
      mineral trioxide aggregate. After 7 to 42 days, the teeth were processed for 
      immunohistochemical and cytochemical staining of fibrillin-1, fibrillin-2, latent 
      TGF-beta-binding protein (LTBP)-1, matrix metalloproteinase-3 (MMP-3), alkaline 
      phosphatase (ALP), and in situ hybridization of fibrillin-1. Pulp tissue slices 
      cultured with beta-glycerophosphate were analyzed for fibrillin-1, fibrillin-2, and 
      ALP with the immunohistochemical/cytochemical staining and quantitative 
      reverse-transcriptase polymerase chain reaction. RESULTS: 
      Fibrillin-1-immunoreactivity was seen until 7 days but turned into undetectable 
      since 14 days in the pulpal area just beneath the exposure site. 
      MMP-3-immunoreaction was transiently detected at 14 days. At 42 days when the 
      mineralized barrier was evident, fibrillin-1-immunoreactivity and fibrillin-1 
      expression remained down-regulated. Fibrillin-2, LTBP-1, and ALP were constantly 
      detected in the fibrillin-1-undetectable area. Pulp slices cultured with 
      beta-glycerophosphate showed mineralization with up-regulation of ALP and 
      down-regulation of fibrillin-1. CONCLUSIONS: Degradation and down-regulation of 
      fibrillin-1 expression took place during the mineralized tissue barrier formation 
      in exposed pulps in vivo and beta-glycerophosphate-induced pulpal mineralization 
      in vitro.
CI  - Copyright (c) 2012 American Association of Endodontists. Published by Elsevier Inc. 
      All rights reserved.
FAU - Yoshiba, Nagako
AU  - Yoshiba N
AD  - Division of Cariology, Operative Dentistry and Endodontics, Department of Oral 
      Health Science, Course for Oral Life Science, Niigata University Graduate School 
      of Medical and Dental Sciences, Niigata, Japan. nagako@dent.niigata-u.ac.jp
FAU - Yoshiba, Kunihiko
AU  - Yoshiba K
FAU - Ohkura, Naoto
AU  - Ohkura N
FAU - Hosoya, Akihiro
AU  - Hosoya A
FAU - Shigetani, Yoshimi
AU  - Shigetani Y
FAU - Yamanaka, Yusuke
AU  - Yamanaka Y
FAU - Izumi, Naoya
AU  - Izumi N
FAU - Nakamura, Hiroaki
AU  - Nakamura H
FAU - Okiji, Takashi
AU  - Okiji T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111027
PL  - United States
TA  - J Endod
JT  - Journal of endodontics
JID - 7511484
RN  - 0 (Aluminum Compounds)
RN  - 0 (Calcium Compounds)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Drug Combinations)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (FBN1 protein, human)
RN  - 0 (FBN2 protein, human)
RN  - 0 (Fibrillin-1)
RN  - 0 (Fibrillin-2)
RN  - 0 (Fibrillins)
RN  - 0 (Glycerophosphates)
RN  - 0 (Latent TGF-beta Binding Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Oxides)
RN  - 0 (Pulp Capping and Pulpectomy Agents)
RN  - 0 (Silicates)
RN  - 0 (mineral trioxide aggregate)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - WWH06G87W6 (beta-glycerophosphoric acid)
MH  - Adolescent
MH  - Adult
MH  - Alkaline Phosphatase/analysis
MH  - Aluminum Compounds/therapeutic use
MH  - Calcification, Physiologic/drug effects
MH  - Calcium Compounds/therapeutic use
MH  - Calcium-Binding Proteins/*analysis
MH  - Cell Differentiation/physiology
MH  - Dental Pulp/drug effects/*pathology
MH  - Dental Pulp Capping/methods
MH  - Dental Pulp Exposure/therapy
MH  - Drug Combinations
MH  - Extracellular Matrix Proteins/*analysis
MH  - Fibrillin-1
MH  - Fibrillin-2
MH  - Fibrillins
MH  - Fibroblasts/drug effects/pathology
MH  - Follow-Up Studies
MH  - Glycerophosphates/pharmacology
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Latent TGF-beta Binding Proteins/analysis
MH  - Matrix Metalloproteinase 3/analysis
MH  - Microfilament Proteins/*analysis
MH  - Odontoblasts/drug effects/pathology
MH  - Oxides/therapeutic use
MH  - Pulp Capping and Pulpectomy Agents/therapeutic use
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Silicates/therapeutic use
MH  - Tissue Culture Techniques
MH  - Wound Healing/physiology
MH  - Young Adult
EDAT- 2012/01/17 06:00
MHDA- 2012/06/22 06:00
CRDT- 2012/01/17 06:00
PHST- 2011/06/07 00:00 [received]
PHST- 2011/09/13 00:00 [revised]
PHST- 2011/09/19 00:00 [accepted]
PHST- 2012/01/17 06:00 [entrez]
PHST- 2012/01/17 06:00 [pubmed]
PHST- 2012/06/22 06:00 [medline]
AID - S0099-2399(11)01089-2 [pii]
AID - 10.1016/j.joen.2011.09.016 [doi]
PST - ppublish
SO  - J Endod. 2012 Feb;38(2):177-84. doi: 10.1016/j.joen.2011.09.016. Epub 2011 Oct 
      27.