PMID- 22244901 OWN - NLM STAT- MEDLINE DCOM- 20120529 LR - 20120207 IS - 1873-2976 (Electronic) IS - 0960-8524 (Linking) VI - 107 DP - 2012 Mar TI - Optimization of clean fractionation processing as a pre-treatment technology for prairie cordgrass. PG - 494-504 LID - 10.1016/j.biortech.2011.12.122 [doi] AB - The main objective of this study was to fractionate prairie cordgrass (PCG) obtaining the highest cellulose digestibility. Following clean fractionation (CF) processing, the PCG lignocellulosic biomass was fractionated into three main building blocks: cellulose, hemicellulose and lignin. Effects of processing factors such as time, temperature, catalyst concentration and organic solvent mixture composition were evaluated. Organic solvent-aqueous mixture contained methyl isobutyl ketone (MIBK), ethanol and water in different proportions. Sulfuric acid was used as a catalyst. In order to evaluate the degree of pre-treatment, enzymatic saccharification was employed on the cellulose fraction obtained from the CF process. Response surface methodology was used for process optimization and statistical analysis. Optimal conditions (39 min, 154 degrees C, 0.69% catalyst and 9% MIBK) resulted in 84% glucose yield and 87% acid insoluble lignin (AIL). CI - Copyright (c) 2011 Elsevier Ltd. All rights reserved. FAU - Brudecki, Grzegorz AU - Brudecki G AD - Department of Agricultural and Biosystems Engineering, South Dakota State University, 1400 N Campus D, Ag. Eng. Building, Brookings, SD 57007, USA. grzegorz.brudecki@sdstate.edu FAU - Cybulska, Iwona AU - Cybulska I FAU - Rosentrater, Kurt AU - Rosentrater K FAU - Julson, James AU - Julson J LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20111230 PL - England TA - Bioresour Technol JT - Bioresource technology JID - 9889523 RN - 11132-73-3 (lignocellulose) RN - 9005-53-2 (Lignin) SB - IM MH - Biomass MH - Catalysis MH - Hydrolysis MH - Lignin MH - *Poaceae EDAT- 2012/01/17 06:00 MHDA- 2012/05/30 06:00 CRDT- 2012/01/17 06:00 PHST- 2011/10/06 00:00 [received] PHST- 2011/12/21 00:00 [revised] PHST- 2011/12/22 00:00 [accepted] PHST- 2012/01/17 06:00 [entrez] PHST- 2012/01/17 06:00 [pubmed] PHST- 2012/05/30 06:00 [medline] AID - S0960-8524(11)01903-1 [pii] AID - 10.1016/j.biortech.2011.12.122 [doi] PST - ppublish SO - Bioresour Technol. 2012 Mar;107:494-504. doi: 10.1016/j.biortech.2011.12.122. Epub 2011 Dec 30.