PMID- 22245116
OWN - NLM
STAT- MEDLINE
DCOM- 20120723
LR  - 20120206
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 145
IP  - 1-2
DP  - 2012 Jan 15
TI  - Monocyte-derived dendritic cells enhance cell proliferation and porcine 
      circovirus type 2 replication in concanavalin A-stimulated swine peripheral blood 
      lymphocytes in vitro.
PG  - 368-78
LID - 10.1016/j.vetimm.2011.12.009 [doi]
AB  - Dendritic cells (DCs) are professional antigen presenting cells cooperating with 
      other immune cells for the activation of innate and adaptive immune responses. 
      The objective of the present study was to investigate the replication activity of 
      porcine circovirus type 2 (PCV2) in DCs and/or lymphocytes during their cross 
      talk and its possible mechanism. Two models were set, herein. Swine blood 
      monocyte (Mo)-derived DCs (MoDCs) or peripheral blood lymphocytes (PBLs) were 
      inoculated with PCV2 prior to their co-cultivation. Bacterial lipopolysaccharide 
      (LPS) and concanavalin A (Con A) were used to stimulate MoDCs and PBLs, 
      respectively. During 6 days of cultivation, a high PCV2 antigen-containing rate 
      without detectable intranuclear signals and a slight but significant increase in 
      the copy number of PCV2 genome were detected in PCV2-inoculated MoDCs. The 
      presence of LPS alone or PCV2-free PBLs, however, had no effect on the location 
      of PCV2 antigens or copy number of PCV2 genome in PCV2-inoculated MoDCs. On the 
      contrary, active PCV2 replication occurred in Con A-stimulated PCV2-inoculated 
      PBLs. When compared with blood Mos, MoDCs induced significantly higher cell 
      proliferation and intensified PCV2 replication in Con A-stimulated 
      PCV2-inoculated PBLs, for which direct contact between MoDCs and lymphocytes was 
      required. Among the cytokines secreted by Con A-activated PBLs, interleukin 
      (IL)-2, but not IL-4 or interferon-gamma, could induce cell proliferation and PCV2 
      replication in PCV2-inoculated PBLs. The findings suggest that although MoDCs 
      support only limited PCV2 replication in themselves, their accessory cell 
      function is required for cell proliferation and PCV2 replication in PCV2-infected 
      lymphocytes.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Lin, Chun-Ming
AU  - Lin CM
AD  - Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, 
      National Taiwan University, Taipei 106, Taiwan, ROC.
FAU - Jeng, Chian-Ren
AU  - Jeng CR
FAU - Hsiao, Shih-Hsuan
AU  - Hsiao SH
FAU - Lee, Yao
AU  - Lee Y
FAU - Tsai, Yi-Chieh
AU  - Tsai YC
FAU - Chia, Mi-Yuan
AU  - Chia MY
FAU - Pang, Victor Fei
AU  - Pang VF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111221
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 11028-71-0 (Concanavalin A)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/drug effects
MH  - Circoviridae Infections/immunology/*veterinary
MH  - Circovirus/*physiology
MH  - Concanavalin A/*pharmacology
MH  - Dendritic Cells/immunology/*physiology
MH  - Flow Cytometry/veterinary
MH  - Fluorescent Antibody Technique, Indirect/veterinary
MH  - Lymphocytes/drug effects/*physiology/virology
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Swine/immunology/virology
MH  - Swine Diseases/immunology/*virology
MH  - Virus Replication/immunology/*physiology
EDAT- 2012/01/17 06:00
MHDA- 2012/07/24 06:00
CRDT- 2012/01/17 06:00
PHST- 2011/07/24 00:00 [received]
PHST- 2011/12/08 00:00 [revised]
PHST- 2011/12/14 00:00 [accepted]
PHST- 2012/01/17 06:00 [entrez]
PHST- 2012/01/17 06:00 [pubmed]
PHST- 2012/07/24 06:00 [medline]
AID - S0165-2427(11)00477-6 [pii]
AID - 10.1016/j.vetimm.2011.12.009 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2012 Jan 15;145(1-2):368-78. doi: 
      10.1016/j.vetimm.2011.12.009. Epub 2011 Dec 21.