PMID- 222456
OWN - NLM
STAT- MEDLINE
DCOM- 19790917
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 16
IP  - 2
DP  - 1979 Feb
TI  - Integration of the DNA of mouse mammary tumor virus in virus-infected normal and 
      neoplastic tissue of the mouse.
PG  - 333-45
AB  - We have used restriction endonucleases which cleave the DNA of mouse mammary 
      tumor virus (MMTV) at one site (Eco RI) and several sites (Pst I, Sac I and Bam 
      HI) to study infection and mammary tumorigenesis in mice. Proviruses acquired 
      during infection of BALB/c mice foster-nursed by virus-producing C3H females can 
      be distinguished from the MMTV proviruses endogenous to uninfected BALB/c mice by 
      the nature of the fragments generated with Pst I and Bam HI. Using this assay, we 
      show that lactating mammary glands as well as mammary tumors from BALB/cfC3H mice 
      have acquired MMTV DNA, and that a minimum of approximately 10% of normal 
      glandular cells can be infected. The new proviruses appear to be linked to 
      cellular DNA of mammary tumors and infected lactating mammary glands within a 
      limited region (0.2 x 10(6) daltons) of the viral DNA; the location of this 
      region, based upon mapping studies with unintegrated MMTV DNA, suggests that the 
      orientation of these proviruses is colinear with linear DNA synthesized in 
      infected cells and thus approximately colinear with the viral RNA. Comparisons of 
      many mammary tumors and studies of lactating mammary glands with a high 
      proportion of independently infected cells indicate that a large number of sites 
      in the cellular genome can accommodate a new provirus; the acquired proviruses 
      are rarely, if ever, found in tandem with each other or with endogenous 
      proviruses. We cannot, however, distinguish between random integration and 
      integration into a large number of preferred sites in the host genome. Since Eco 
      RI and Bam HI cleavage of DNA from each mammary tumor generates a unique set of 
      viral-specific fragments, we propose that the tumors are composed principally of 
      cells derived from a subset of the many infected cells in a mammary gland; this 
      proposal is supported by our finding that Eco RI digestion of DNA from several 
      transplants of a primary tumor yields the pattern characteristic of the primary 
      tumor.
FAU - Cohen, J C
AU  - Cohen JC
FAU - Shank, P R
AU  - Shank PR
FAU - Morris, V L
AU  - Morris VL
FAU - Cardiff, R
AU  - Cardiff R
FAU - Varmus, H E
AU  - Varmus HE
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA, Viral)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *Cell Transformation, Viral
MH  - Clone Cells/microbiology
MH  - DNA/metabolism
MH  - DNA Restriction Enzymes/metabolism
MH  - DNA, Neoplasm/*metabolism
MH  - DNA, Viral/*metabolism
MH  - Female
MH  - Lactation
MH  - Mammary Glands, Animal/*microbiology
MH  - Mammary Neoplasms, Experimental/*microbiology
MH  - Mammary Tumor Virus, Mouse/*metabolism
MH  - Mice
MH  - Pregnancy
EDAT- 1979/02/01 00:00
MHDA- 1979/02/01 00:01
CRDT- 1979/02/01 00:00
PHST- 1979/02/01 00:00 [pubmed]
PHST- 1979/02/01 00:01 [medline]
PHST- 1979/02/01 00:00 [entrez]
AID - 0092-8674(79)90010-2 [pii]
AID - 10.1016/0092-8674(79)90010-2 [doi]
PST - ppublish
SO  - Cell. 1979 Feb;16(2):333-45. doi: 10.1016/0092-8674(79)90010-2.