PMID- 22249002 OWN - NLM STAT- MEDLINE DCOM- 20120622 LR - 20120402 IS - 1879-1220 (Electronic) IS - 0960-0760 (Linking) VI - 130 IP - 1-2 DP - 2012 May TI - Cloning, characterization, and expression analysis of a putative 17 beta-hydroxysteroid dehydrogenase 11 in the abalone, Haliotis diversicolor supertexta. PG - 57-63 LID - 10.1016/j.jsbmb.2011.12.013 [doi] AB - The 17-beta-hydroxysteroid dehydrogenases (17beta-HSDs) are key enzymes for sex steroid biosynthesis. To date, relatively little is known about the presence and function of 17beta-HSDs in marine gastropods. In the present study, a cDNA sequence encoding putative 17beta-HSD type 11 (17beta-HSD-11) was identified in marine abalone (Haliotis diversicolor supertexta). The full-length cDNA contains 1058bp, including an open reading frame (ORF) of 900bp that encodes a protein of 299 amino acids. Comparative structural analysis revealed that abalone 17beta-HSD-11 shares relatively high homology with other 17b-HSD-11 hormologues, and a lesser degree of amino acid identity with other forms of 17b-HSD, especially in the functional domains, including the cofactor binding domain (TGxxxGxG) and catalytic site (YxxSK). Phylogenetic analysis showed that abalone 17beta-HSD-11 belongs to the short-chain dehydrogenase/reductase (SDR) family. Functional analysis following transient transfection of the ORF into human embryonic kidney-293 (HEK-293) cells indicated that abalone 17beta-HSD-11 has the ability to convert 5alpha-androstane-3alpha,17beta-diol (3alpha-diol) to androsterone (A) and testosterone (T) to androstenedione (4A). Expression analysis in vivo demonstrated that abalone 17beta-HSD-11 is differentially expressed during three stages (non-reproductive, reproductive, and post-reproductive). Taken together, these results indicate that ab-17beta-HSD-11 is an SDR family member with a potential role in steroid regulation during the reproductive stage. CI - Copyright (c) 2011 Elsevier Ltd. All rights reserved. FAU - Zhai, Hong-ning AU - Zhai HN AD - School of Life Sciences, Tsinghua University, Beijing, PR China. FAU - Zhou, Jin AU - Zhou J FAU - Cai, Zhong-hua AU - Cai ZH LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20120112 PL - England TA - J Steroid Biochem Mol Biol JT - The Journal of steroid biochemistry and molecular biology JID - 9015483 RN - 0 (DNA, Complementary) RN - EC 1.1.- (17-Hydroxysteroid Dehydrogenases) SB - IM MH - 17-Hydroxysteroid Dehydrogenases/*genetics/metabolism MH - Animals MH - Cloning, Molecular MH - DNA, Complementary/chemistry MH - Gastropoda/*enzymology/genetics/metabolism MH - HEK293 Cells MH - Humans MH - Open Reading Frames MH - Phylogeography MH - Transfection EDAT- 2012/01/18 06:00 MHDA- 2012/06/23 06:00 CRDT- 2012/01/18 06:00 PHST- 2011/07/09 00:00 [received] PHST- 2011/12/17 00:00 [revised] PHST- 2011/12/19 00:00 [accepted] PHST- 2012/01/18 06:00 [entrez] PHST- 2012/01/18 06:00 [pubmed] PHST- 2012/06/23 06:00 [medline] AID - S0960-0760(11)00255-X [pii] AID - 10.1016/j.jsbmb.2011.12.013 [doi] PST - ppublish SO - J Steroid Biochem Mol Biol. 2012 May;130(1-2):57-63. doi: 10.1016/j.jsbmb.2011.12.013. Epub 2012 Jan 12.