PMID- 22249604
OWN - NLM
STAT- MEDLINE
DCOM- 20120928
LR  - 20220311
IS  - 1573-0832 (Electronic)
IS  - 0301-486X (Print)
IS  - 0301-486X (Linking)
VI  - 174
IP  - 1
DP  - 2012 Jul
TI  - Synthetic peptides mimic gp75 from Paracoccidioides brasiliensis in the diagnosis 
      of paracoccidioidomycosis.
PG  - 1-10
LID - 10.1007/s11046-011-9518-3 [doi]
AB  - Paracoccidioidomycosis (PCM) is a systemic granulomatous disease, endemic in 
      Latin America, caused by the thermal dimorphic fungus Paracoccidioides 
      brasiliensis. Although some fungal antigens have already been characterized and 
      used for serological diagnosis, cross-reactions have been frequently observed. 
      Thus, the examination of fungal forms in clinical specimens or isolation of P. 
      brasiliensis by culture is still the most frequent method for the diagnosis of 
      this mycosis. In this study, a random peptide phage display library was used to 
      select mimotopes of P. brasiliensis, which were employed as antigens in an 
      indirect enzyme-linked immunosorbent assay. The protective monoclonal antibody 
      against experimental PCM (anti-gp75) was used as molecular target to screen a 
      phage display library. That approach led to a synthetic peptide named P2, which 
      was synthesized and tested against PCM patients' sera to check whether it was 
      recognized. There was significant recognition of P2 by sera of untreated PCM 
      patients when compared with normal human sera. Sera from treated PCM group, 
      patients with other mycosis or co-infected with HIV had much lower recognition of 
      P2 than untreated patient group. The test showed a sensitivity of 100 and 94.59% 
      of specificity in relation to human sera control. These data indicate a potential 
      use of P2 as diagnostic tool in PCM. Its application for serological diagnosis of 
      PCM may contribute to the development and standardization of simpler, faster and 
      highly reproducible immunodiagnostic tests at low cost.
FAU - Caldini, Camila Pistelli
AU  - Caldini CP
AD  - Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal 
      de Sao Paulo-Escola Paulista de Medicina, Disciplina de Imunologia, Rua Botucatu, 
      862, 4 masculine andar, Sao Paulo, 04023-900, Brazil.
FAU - Xander, Patricia
AU  - Xander P
FAU - Kioshima, Erika Seki
AU  - Kioshima ES
FAU - Bachi, Andre Luis Lacerda
AU  - Bachi AL
FAU - de Camargo, Zoilo Pires
AU  - de Camargo ZP
FAU - Mariano, Mario
AU  - Mariano M
FAU - Lopes, Jose Daniel
AU  - Lopes JD
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120117
PL  - Netherlands
TA  - Mycopathologia
JT  - Mycopathologia
JID - 7505689
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Peptide Library)
RN  - 0 (Peptides)
SB  - IM
MH  - Animals
MH  - Antibodies, Fungal/*blood
MH  - Antibodies, Monoclonal/immunology/isolation & purification
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mycology/*methods
MH  - Paracoccidioidomycosis/*diagnosis
MH  - Peptide Library
MH  - *Peptides/isolation & purification
MH  - Sensitivity and Specificity
MH  - Serologic Tests/methods
PMC - PMC3368115
EDAT- 2012/01/18 06:00
MHDA- 2012/09/29 06:00
PMCR- 2012/01/17
CRDT- 2012/01/18 06:00
PHST- 2011/08/18 00:00 [received]
PHST- 2011/12/06 00:00 [accepted]
PHST- 2012/01/18 06:00 [entrez]
PHST- 2012/01/18 06:00 [pubmed]
PHST- 2012/09/29 06:00 [medline]
PHST- 2012/01/17 00:00 [pmc-release]
AID - 9518 [pii]
AID - 10.1007/s11046-011-9518-3 [doi]
PST - ppublish
SO  - Mycopathologia. 2012 Jul;174(1):1-10. doi: 10.1007/s11046-011-9518-3. Epub 2012 
      Jan 17.