PMID- 22251575
OWN - NLM
STAT- MEDLINE
DCOM- 20121029
LR  - 20220330
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 57
IP  - 6
DP  - 2012 Jun
TI  - Effects of alendronate on human osteoblast-like MG63 cells and matrix 
      metalloproteinases.
PG  - 728-36
LID - 10.1016/j.archoralbio.2011.12.007 [doi]
AB  - OBJECTIVE: The objective of this study was to examine the effects of alendronate 
      on the expression and activity of matrix metalloproteinases (MMPs) and the 
      expression of the tissue inhibitors of MMPs (TIMPs) from human osteoblast-like 
      MG63 cells. MATERIALS AND METHODS: MG63 cells were exposed to various 
      concentrations of alendronate. Cell proliferation and cytotoxicity were evaluated 
      by water-soluble tetrazolium-1 and lactate dehydrogenase, respectively. 
      MG63-mediated collagen degradation was assessed utilising Type I collagen assays. 
      Conditioned media and membrane extracts were collected for Western blot analyses 
      of select MMPs and TIMPs. Gelatin zymography gels were incubated with alendronate 
      to assess its effects on MMP-2 activity. RESULTS: Alendronate affected MG63 
      proliferation and cytotoxicity at concentrations equal to/or greater than 10(-5) 
      M (all p < 0.05). There were no significant differences in the collagen degrading 
      ability of treated cells at non-toxic levels vs. untreated cells. Alendronate had 
      no effects on the expression of MMP-2 or MT1-MMP (membrane type-1 MMP) in the 
      conditioned media or membrane extracts, and of MMP-1 or TIMP-2 in the conditioned 
      media. TIMP-2 in the membrane extracts was not detectable. MMP-2 activity in the 
      zymograms was inhibited by 10(-3) and 10(-2) M alendronate. CONCLUSION: 
      Alendronate at 10(-5) M or higher was toxic to the cells. Alendronate at 10(-8) 
      to 10(-6) M did not alter the expression of MMP-1, MMP-2, MT1-MMP or TIMP-2, as 
      well as did not alter collagen degradation. Alendronate inhibited MMP-2 activity 
      at 10(-3) and 10(-2) M in the zymograms. In conclusion, non-toxic levels of 
      alendronate (10(-8) to 10(-6) M) did not alter MMP expression in MG63 cells or 
      inhibit MMP-2 activity.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Sun, Jun
AU  - Sun J
AD  - Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, 
      IN 46202, USA.
FAU - Song, Fengyu
AU  - Song F
FAU - Zhang, Weiping
AU  - Zhang W
FAU - Sexton, Brent E
AU  - Sexton BE
FAU - Windsor, L Jack
AU  - Windsor LJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120116
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Bone Density Conservation Agents)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - 9007-34-5 (Collagen)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - X1J18R4W8P (Alendronate)
SB  - IM
MH  - Alendronate/*pharmacology
MH  - Analysis of Variance
MH  - Blotting, Western
MH  - Bone Density Conservation Agents/*pharmacology
MH  - Cell Line
MH  - Cell Proliferation
MH  - Collagen/metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Matrix Metalloproteinase 2/*drug effects/*metabolism
MH  - Osteoblasts/*drug effects
MH  - Tissue Inhibitor of Metalloproteinase-1/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-2/metabolism
EDAT- 2012/01/19 06:00
MHDA- 2012/10/30 06:00
CRDT- 2012/01/19 06:00
PHST- 2011/07/13 00:00 [received]
PHST- 2011/12/16 00:00 [revised]
PHST- 2011/12/19 00:00 [accepted]
PHST- 2012/01/19 06:00 [entrez]
PHST- 2012/01/19 06:00 [pubmed]
PHST- 2012/10/30 06:00 [medline]
AID - S0003-9969(11)00423-7 [pii]
AID - 10.1016/j.archoralbio.2011.12.007 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2012 Jun;57(6):728-36. doi: 10.1016/j.archoralbio.2011.12.007. 
      Epub 2012 Jan 16.