PMID- 22254128
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20120823
LR  - 20211021
IS  - 2090-0058 (Electronic)
IS  - 2090-004X (Print)
IS  - 2090-004X (Linking)
VI  - 2011
DP  - 2011
TI  - Inflammatory and angiogenic protein detection in the human vitreous: cytometric 
      bead assay.
PG  - 459251
LID - 10.1155/2011/459251 [doi]
LID - 459251
AB  - Introduction. To evaluate clinical feasibility and reproducibility of cytometric 
      bead assay (CBA) in nondiluted vitreous samples of patients with age-related 
      macular degeneration (ARMD), diabetic macular edema (DME), and central retinal 
      vein occlusion (CRVO). Methods. Twelve patients from a single clinics day 
      qualified for intravitreal injections (ARMD n = 6, DME n = 3, CRVO n = 3) and 
      underwent a combination treatment including a single-site 23 gauge core 
      vitrectomy which yielded a volume of 0.6 mL undiluted vitreous per patient. 
      Interleukin-6 (IL-6), vascular endothelial growth factor isoform A (VEGF-A), and 
      monocyte chemo-attractant protein-1 (MCP-1) were assessed directly from 0.3 mL at 
      the same day (fresh samples). To assess the reproducibility 0.3 ml were frozen 
      for 60 days at -80 degrees , on which the CBA was repeated (frozen samples). Results. In 
      the fresh samples IL-6 was highest in CRVO (median IL-6 55.8 pg/mL) > DME (50.6) 
      > ARMD (3.1). Highest VEGF was measured in CRVO (447.4) > DME (3.9) > ARMD (2.0). 
      MCP-1 was highest in CRVO (595.7) > AMD (530.8) > DME (178). The CBA 
      reproducibility after frozen storage was examined to be most accurate for MCP1 (P 
      = 0.91) > VEGF (P = 0.68) > IL-6 (P = 0.49). Conclusions. CBA is an innovative, 
      fast determining, and reliable technology to analyze proteins in fluids, like the 
      undiluted vitreous, which is important to better understand ocular 
      pathophysiology and pharmacology. There is no influence of intermittent storage 
      at -80 degrees  for the reproducibility of the CBA.
FAU - Koss, M J
AU  - Koss MJ
AD  - Section of Vitreo-Retinal Surgery, Department of Ophthalmology, Hospital of the 
      Goethe University, Goethe University, 60590 Frankfurt am Main, Germany.
FAU - Pfister, M
AU  - Pfister M
FAU - Koch, F H
AU  - Koch FH
LA  - eng
PT  - Journal Article
DEP - 20111227
PL  - United States
TA  - J Ophthalmol
JT  - Journal of ophthalmology
JID - 101524199
PMC - PMC3255300
EDAT- 2012/01/19 06:00
MHDA- 2012/01/19 06:01
PMCR- 2011/12/27
CRDT- 2012/01/19 06:00
PHST- 2011/07/13 00:00 [received]
PHST- 2011/11/22 00:00 [accepted]
PHST- 2012/01/19 06:00 [entrez]
PHST- 2012/01/19 06:00 [pubmed]
PHST- 2012/01/19 06:01 [medline]
PHST- 2011/12/27 00:00 [pmc-release]
AID - 10.1155/2011/459251 [doi]
PST - ppublish
SO  - J Ophthalmol. 2011;2011:459251. doi: 10.1155/2011/459251. Epub 2011 Dec 27.