PMID- 22256298
OWN - NLM
STAT- MEDLINE
DCOM- 20120531
LR  - 20200928
IS  - 2694-0604 (Electronic)
IS  - 2375-7477 (Linking)
VI  - 2011
DP  - 2011
TI  - Towards a cellular multi-parameter analysis platform: fluorescence in situ 
      hybridization (FISH) on microhole-array chips.
PG  - 8408-11
LID - 10.1109/IEMBS.2011.6092074 [doi]
AB  - Highly-sensitive analysis systems based on cellular multi-parameter are needed in 
      the diagnostics. Therefore we improved our previously developed chip platform for 
      another additional analysis method, the fluorescence in situ hybridization. 
      Fluorescence in situ hybridization (FISH) is a technique used in the diagnostics 
      to determine the localization and the presence or absence of specific DNA 
      sequence. To improve this labor- and cost-intensive method, we reduced the assay 
      consumption by a factor of 5 compared to the standard protocol. Microhole chips 
      were used for making the cells well addressable. The chips were fabricated by 
      semiconductor technology on the basis of a Silicon wafer with a thin deposited 
      silicon nitride layer (Si(3)N(4)). Human retina pigment epithelia (ARPE-19) cells 
      were arrayed on 5-mum holes of a 35 x 35 microhole-array by a gently negative 
      differential pressure of around 5 mbar. After 3 hours of incubation the cells 
      were attached to the chip and the FISH protocol was applied to the positioned 
      cells. A LabView software was developed to simplify the analysis. The software 
      automatically counts the number of dots (positive labeled chromosome regions) as 
      well as the distance between adjacent dots. Our developed platform reduces the 
      assay consumption and the labor time. Furthermore, during the 3 hours of 
      incubation non-invasive or minimal-invasive methods like Raman- and 
      impedance-spectroscopy can be applied.
FAU - Kurz, Christian M
AU  - Kurz CM
AD  - Department Biomedical Microsystems, Fraunhofer Insitute for Biomedical 
      Engineering.
FAU - Moosdijk, Stefan V D
AU  - Moosdijk SV
FAU - Thielecke, Hagen
AU  - Thielecke H
FAU - Velten, Thomas
AU  - Velten T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Annu Int Conf IEEE Eng Med Biol Soc
JT  - Annual International Conference of the IEEE Engineering in Medicine and Biology 
      Society. IEEE Engineering in Medicine and Biology Society. Annual International 
      Conference
JID - 101763872
RN  - 0 (Xanthenes)
RN  - 82354-19-6 (Texas red)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Cell Line
MH  - Fluorescein-5-isothiocyanate/chemistry
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Light
MH  - Microfluidic Analytical Techniques/*methods
MH  - Microscopy, Fluorescence
MH  - Software
MH  - Xanthenes/chemistry
EDAT- 2012/01/19 06:00
MHDA- 2012/06/01 06:00
CRDT- 2012/01/19 06:00
PHST- 2012/01/19 06:00 [entrez]
PHST- 2012/01/19 06:00 [pubmed]
PHST- 2012/06/01 06:00 [medline]
AID - 10.1109/IEMBS.2011.6092074 [doi]
PST - ppublish
SO  - Annu Int Conf IEEE Eng Med Biol Soc. 2011;2011:8408-11. doi: 
      10.1109/IEMBS.2011.6092074.