PMID- 22256790
OWN - NLM
STAT- MEDLINE
DCOM- 20120808
LR  - 20120419
IS  - 1462-5822 (Electronic)
IS  - 1462-5814 (Linking)
VI  - 14
IP  - 5
DP  - 2012 May
TI  - Coronin-1a inhibits autophagosome formation around Mycobacterium 
      tuberculosis-containing phagosomes and assists mycobacterial survival in 
      macrophages.
PG  - 710-27
LID - 10.1111/j.1462-5822.2012.01754.x [doi]
AB  - Mycobacterium tuberculosis is an intracellular bacterium that can survive within 
      macrophages. Such survival is potentially associated with Coronin-1a (Coro1a). We 
      investigated the mechanism by which Coro1a promotes the survival of M. 
      tuberculosis in macrophages and found that autophagy was involved in the 
      inhibition of mycobacterial survival in Coro1a knock-down (KD) macrophages. 
      Fluorescence microscopy and immunoblot analyses revealed that LC3, a 
      representative autophagic protein, was recruited to M. tuberculosis-containing 
      phagosomes in Coro1a KD macrophages. Thin-section electron microscopy 
      demonstrated that bacilli were surrounded by the multiple membrane structures in 
      Coro1a KD macrophages. The proportion of LC3-positive mycobacterial phagosomes 
      colocalized with p62/SQSTM1, ubiquitin or LAMP1 increased in Coro1a KD 
      macrophages during infection. These results demonstrate the formation of 
      autophagosomes around M. tuberculosis in Coro1a KD macrophages. Phosphorylation 
      of p38 mitogen-activated protein kinase (MAPK) was induced in response to M. 
      tuberculosis infection in Coro1a KD macrophages, suggesting that Coro1a blocks 
      the activation of the p38 MAPK pathway involved in autophagosome formation. LC3 
      recruitment to M. tuberculosis-containing phagosomes was also observed in Coro1a 
      KD alveolar or bone marrow-derived macrophages. These results suggest that Coro1a 
      inhibits autophagosome formation in alveolar macrophages, thereby facilitating M. 
      tuberculosis survival within the lung.
CI  - (c) 2012 Blackwell Publishing Ltd.
FAU - Seto, Shintaro
AU  - Seto S
AD  - Department of Infectious Diseases, Hamamatsu University School of Medicine, 
      1-20-1 Handa-yama, Higashi-ku, Hamamatsu 431-3192, Japan. s-seto@hama-med.ac.jp
FAU - Tsujimura, Kunio
AU  - Tsujimura K
FAU - Koide, Yukio
AU  - Koide Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120214
PL  - India
TA  - Cell Microbiol
JT  - Cellular microbiology
JID - 100883691
RN  - 0 (Microfilament Proteins)
RN  - 145420-64-0 (coronin proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Gene Knockdown Techniques
MH  - *Host-Pathogen Interactions
MH  - Immunoblotting
MH  - Macrophages/*microbiology/ultrastructure
MH  - Mice
MH  - Microbial Viability
MH  - Microfilament Proteins/genetics/*metabolism
MH  - Microscopy, Electron
MH  - Microscopy, Fluorescence
MH  - Mycobacterium tuberculosis/*pathogenicity/ultrastructure
MH  - Phagosomes/*microbiology/ultrastructure
EDAT- 2012/01/20 06:00
MHDA- 2012/08/09 06:00
CRDT- 2012/01/20 06:00
PHST- 2012/01/20 06:00 [entrez]
PHST- 2012/01/20 06:00 [pubmed]
PHST- 2012/08/09 06:00 [medline]
AID - 10.1111/j.1462-5822.2012.01754.x [doi]
PST - ppublish
SO  - Cell Microbiol. 2012 May;14(5):710-27. doi: 10.1111/j.1462-5822.2012.01754.x. 
      Epub 2012 Feb 14.