PMID- 22257110
OWN - NLM
STAT- MEDLINE
DCOM- 20120625
LR  - 20120314
IS  - 1398-9995 (Electronic)
IS  - 0105-4538 (Linking)
VI  - 67
IP  - 4
DP  - 2012 Apr
TI  - Implications of nasopharynx-associated lymphoid tissue (NALT) in the development 
      of allergic responses in an allergic rhinitis mouse model.
PG  - 502-9
LID - 10.1111/j.1398-9995.2011.02782.x [doi]
AB  - BACKGROUND: Nasopharynx-associated lymphoid tissue (NALT) serves as an important 
      inductive site for mucosal immunity in the upper respiratory tract. Despite its 
      importance in the mucosal immune system, little is known regarding the role of 
      NALT in airway allergic immune responses. We aimed to elucidate the role of NALT 
      in the induction of upper airway allergic responses in a mouse model. METHODS: 
      Inhibitor of DNA binding/differentiation 2 (Id2)(-/-) and Id2(+/-) mice was 
      exposed to the ovalbumin (OVA)-induced allergic rhinitis model, because the 
      former resulted in the NALT deficiency. The allergic parameters, such as allergic 
      symptoms, serum OVA-specific immunoglobulin E (IgE) levels, eosinophil 
      infiltration, and cytokine profiles in the nasal mucosa, were compared between 
      Id2(-/-) and Id2(+/-) groups. RESULTS: NALT-null, Id2(-/-) mice displayed 
      significantly lower allergic responses compared with Id2(+/-) mice, as 
      demonstrated by lower levels of allergic symptoms, serum OVA-specific IgE, 
      eosinophilic infiltration, and local Th2 cytokine transcriptions. To determine 
      which of two factors, that is, the absence of NALT or the alteration of 
      immunocompetent cell populations caused by the Id2 deficiency, has a larger 
      effect on the attenuated allergic immune responses in Id2(-/-) mice, lethally 
      irradiated Id2(-/-) mice were engrafted with C57BL/6 wild-type bone marrow cells 
      and showed still significantly lower allergic immune responses compared with 
      equally treated Id2(+/-) mice. In addition, IgE class switch 
      recombination-associated molecules, such as epsilon immunoglobulin heavy-chain germline 
      gene transcript, epsilon mRNA, and activation-induced cytidine deaminase mRNA, were 
      detected in NALT from OVA-sensitized wild-type mice. CONCLUSION: These results 
      show the critical role of NALT for the induction of allergic responses in the 
      upper airway at least in part by means of class switching to IgE in situ.
CI  - (c) 2012 John Wiley & Sons A/S.
FAU - Kim, D-Y
AU  - Kim DY
AD  - Department of Otorhinolaryngology, Seoul National University College of Medicine, 
      Chongno-gu, Seoul, Korea. kiyono@ims.u-tokyo.ac.jp
FAU - Fukuyama, S
AU  - Fukuyama S
FAU - Nagatake, T
AU  - Nagatake T
FAU - Takamura, K
AU  - Takamura K
FAU - Kong, I G
AU  - Kong IG
FAU - Yokota, Y
AU  - Yokota Y
FAU - Lee, C H
AU  - Lee CH
FAU - Kiyono, H
AU  - Kiyono H
LA  - eng
PT  - Journal Article
DEP - 20120119
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Cytokines)
RN  - 0 (Idb2 protein, mouse)
RN  - 0 (Inhibitor of Differentiation Protein 2)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Cytokines/analysis/biosynthesis/immunology
MH  - Disease Models, Animal
MH  - Hypersensitivity/*immunology
MH  - Immunity, Mucosal/*immunology
MH  - Immunoglobulin Class Switching/immunology
MH  - Immunoglobulin E/immunology
MH  - Inhibitor of Differentiation Protein 2/deficiency
MH  - Lymphoid Tissue/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Nasopharynx/*immunology
MH  - Real-Time Polymerase Chain Reaction
MH  - Rhinitis/*immunology
EDAT- 2012/01/20 06:00
MHDA- 2012/06/26 06:00
CRDT- 2012/01/20 06:00
PHST- 2011/12/10 00:00 [accepted]
PHST- 2012/01/20 06:00 [entrez]
PHST- 2012/01/20 06:00 [pubmed]
PHST- 2012/06/26 06:00 [medline]
AID - 10.1111/j.1398-9995.2011.02782.x [doi]
PST - ppublish
SO  - Allergy. 2012 Apr;67(4):502-9. doi: 10.1111/j.1398-9995.2011.02782.x. Epub 2012 
      Jan 19.