PMID- 22259066 OWN - NLM STAT- MEDLINE DCOM- 20120611 LR - 20220316 IS - 1945-7197 (Electronic) IS - 0021-972X (Linking) VI - 97 IP - 4 DP - 2012 Apr TI - Evidence of a combined cytotoxic thyroglobulin and thyroperoxidase epitope-specific cellular immunity in Hashimoto's thyroiditis. PG - 1347-54 LID - 10.1210/jc.2011-2178 [doi] AB - CONTEXT: Hashimoto's thyroiditis (HT) is a common autoimmune disease leading to thyroid destruction due to lymphocytic infiltration. Only rare data are available regarding the recognition of specific cellular antigens, e.g. of thyroperoxidase (TPO) and thyroglobulin (Tg). OBJECTIVE: The aim of this study was to quantify and characterize TPO- and Tg-epitope-specific CD8-positive T cells of HT patients. DESIGN: Six different human leukocyte antigen (HLA)-A2 restricted, TPO- or Tg-specific tetramers were synthesized and used for measuring CD8-positive T cells in HT patients and controls. RESULTS: The frequency of peripheral TPO- and Tg-specific CD8-positive T cells was significantly higher in HLA-A2-positive HT patients (2.8 +/- 9.5%) compared with HLA-A2-negative HT patients (0.5 +/- 0.7%), HLA-A2-positive nonautoimmune goiter patients (0.2 +/- 0.4%), and HLA-A2-positive healthy controls (0.1 +/- 0.2%). The frequency of Tg-specific T cells (3.0%) was very similar to those of TPO-specific CD8-positive T cells (2.9%). Subgroup analyses revealed a steady increase of the number of epitope-specific CD8-positive T cells from 0.6 +/- 1.0% at initial diagnosis up to 9.4 +/- 18.3% in patients with long-lasting disease. Analyses of the number of thyroid-infiltrating cells as well as the cytotoxic capacity revealed a similar picture for TPO- and Tg-specific T cells. CONCLUSION: We here report for the first time that both antigens, TPO and Tg, are recognized by CD8-positive T cells and are involved in the thyroid destruction process leading to clinical disease manifestation. FAU - Ehlers, Margret AU - Ehlers M AD - Department of Endocrinology, Diabetes, and Rheumatology, University Hospital Duesseldorf, Moorenstrasse 5, D-40225 Duesseldorf, Germany. FAU - Thiel, Annette AU - Thiel A FAU - Bernecker, Christian AU - Bernecker C FAU - Porwol, Dominika AU - Porwol D FAU - Papewalis, Claudia AU - Papewalis C FAU - Willenberg, Holger S AU - Willenberg HS FAU - Schinner, Sven AU - Schinner S FAU - Hautzel, Hubertus AU - Hautzel H FAU - Scherbaum, Werner A AU - Scherbaum WA FAU - Schott, Matthias AU - Schott M LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20120118 PL - United States TA - J Clin Endocrinol Metab JT - The Journal of clinical endocrinology and metabolism JID - 0375362 RN - 0 (Autoantibodies) RN - 0 (Autoantigens) RN - 0 (Epitopes) RN - 0 (HLA-A2 Antigen) RN - 0 (Iron-Binding Proteins) RN - 0 (anti-thyroglobulin) RN - 9010-34-8 (Thyroglobulin) RN - EC 1.11.1.7 (TPO protein, human) RN - EC 1.11.1.8 (Iodide Peroxidase) SB - IM MH - Adult MH - Aged MH - Antibody Specificity MH - *Antibody-Dependent Cell Cytotoxicity MH - Autoantibodies/*analysis/chemistry MH - Autoantigens/chemistry MH - Biopsy, Fine-Needle MH - CD8-Positive T-Lymphocytes/immunology/metabolism/pathology MH - Epitopes/analysis/chemistry MH - Female MH - Goiter/immunology/metabolism/pathology MH - HLA-A2 Antigen/metabolism MH - Hashimoto Disease/*immunology/metabolism/pathology MH - Humans MH - *Immunity, Cellular MH - Iodide Peroxidase/*antagonists & inhibitors/chemistry MH - Iron-Binding Proteins/*antagonists & inhibitors/chemistry MH - Male MH - Middle Aged MH - Thyroglobulin/*antagonists & inhibitors/chemistry MH - Thyroid Gland/*immunology/pathology MH - Young Adult EDAT- 2012/01/20 06:00 MHDA- 2012/06/12 06:00 CRDT- 2012/01/20 06:00 PHST- 2012/01/20 06:00 [entrez] PHST- 2012/01/20 06:00 [pubmed] PHST- 2012/06/12 06:00 [medline] AID - jc.2011-2178 [pii] AID - 10.1210/jc.2011-2178 [doi] PST - ppublish SO - J Clin Endocrinol Metab. 2012 Apr;97(4):1347-54. doi: 10.1210/jc.2011-2178. Epub 2012 Jan 18.