PMID- 22260235
OWN - NLM
STAT- MEDLINE
DCOM- 20130107
LR  - 20211021
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 16
IP  - 9
DP  - 2012 Sep
TI  - Ethanol increases phosphate-mediated mineralization and osteoblastic 
      transformation of vascular smooth muscle cells.
PG  - 2219-26
LID - 10.1111/j.1582-4934.2012.01533.x [doi]
AB  - Vascular calcification is implicated in the pathogenesis of atherosclerosis, 
      diabetes and chronic kidney disease. Human vascular smooth muscle cells (HSMCs) 
      undergo mineralization in response to elevated levels of inorganic phosphate (Pi) 
      in an active and well-regulated process. This process involves increased activity 
      of alkaline phosphatase and increased expression of core binding factor alpha-1 
      (CBF-alpha1), a bone-specific transcription factor, with the subsequent induction of 
      osteocalcin. It has been shown that heavy alcohol consumption is associated with 
      greater calcification in coronary arteries. The goal of our study was to examine 
      whether ethanol alters mineralization of HSMCs provoked by high Pi. Exposure of 
      HSMCs to ethanol increased extracellular matrix calcification in a dose 
      responsive manner, providing a significant additional calcium deposition at 
      concentrations of >/=60 mmol/l. HSMC calcification was accompanied by further 
      enhancement in alkaline phosphatase activity. Ethanol also provoked a significant 
      increase in the synthesis of osteocalcin. Moreover, in cells challenged with 
      ethanol the expression of CBF-alpha1, a transcription factor involved in the 
      regulation of osteoblastic transformation of HSMCs, was elevated. The observed 
      effects of ethanol were not due to alterations of phosphate uptake by HSMCs. We 
      conclude that ethanol enhances Pi-mediated human vascular smooth muscle 
      calcification and transition of these cells into osteoblast-like cells.
CI  - (c) 2012 The Authors Journal of Cellular and Molecular Medicine (c) 2012 Foundation 
      for Cellular and Molecular Medicine/Blackwell Publishing Ltd.
FAU - Oros, Melinda
AU  - Oros M
AD  - Department of Biochemistry and Molecular Biology, Medical and Health Science 
      Center, University of Debrecen, Debrecen, Hungary.
FAU - Zavaczki, Erzsebet
AU  - Zavaczki E
FAU - Vadasz, Csaba
AU  - Vadasz C
FAU - Jeney, Viktoria
AU  - Jeney V
FAU - Tosaki, Arpad
AU  - Tosaki A
FAU - Lekli, Istvan
AU  - Lekli I
FAU - Balla, Gyorgy
AU  - Balla G
FAU - Nagy, Laszlo
AU  - Nagy L
FAU - Balla, Jozsef
AU  - Balla J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Phosphates)
RN  - 0 (RUNX2 protein, human)
RN  - 0 (Transcription Factors)
RN  - 104982-03-8 (Osteocalcin)
RN  - 3K9958V90M (Ethanol)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Calcinosis/*pathology
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Core Binding Factor Alpha 1 Subunit/genetics/metabolism
MH  - Ethanol/*adverse effects
MH  - Extracellular Matrix/metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Muscle, Smooth, Vascular/*cytology/drug effects/pathology
MH  - Myocytes, Smooth Muscle/cytology/*drug effects/metabolism
MH  - Osteoblasts/*cytology/drug effects/metabolism
MH  - Osteocalcin/genetics/metabolism
MH  - Phosphates/*analysis/pharmacokinetics
MH  - Transcription Factors/genetics/metabolism
PMC - PMC3822991
EDAT- 2012/01/21 06:00
MHDA- 2013/01/08 06:00
PMCR- 2012/09/01
CRDT- 2012/01/21 06:00
PHST- 2012/01/21 06:00 [entrez]
PHST- 2012/01/21 06:00 [pubmed]
PHST- 2013/01/08 06:00 [medline]
PHST- 2012/09/01 00:00 [pmc-release]
AID - 10.1111/j.1582-4934.2012.01533.x [doi]
PST - ppublish
SO  - J Cell Mol Med. 2012 Sep;16(9):2219-26. doi: 10.1111/j.1582-4934.2012.01533.x.