PMID- 22261049
OWN - NLM
STAT- MEDLINE
DCOM- 20120509
LR  - 20211021
IS  - 1542-0086 (Electronic)
IS  - 0006-3495 (Print)
IS  - 0006-3495 (Linking)
VI  - 101
IP  - 11
DP  - 2011 Dec 7
TI  - Measuring traction forces of motile dendritic cells on micropost arrays.
PG  - 2620-8
LID - 10.1016/j.bpj.2011.09.022 [doi]
AB  - Dendritic cells (DCs) migrate from sites of inflammation to secondary lymphoid 
      organs where they initiate the adaptive immune response. Although motility is 
      essential to DC function, the mechanisms by which they migrate are not 
      fully understood. We incorporated micropost array detectors into a microfluidic 
      gradient generator to develop what we consider to be a novel method for probing 
      low magnitude traction forces during directional migration. We found migration of 
      primary murine DCs is driven by short-lived traction stresses at the leading edge 
      or filopodia. The traction forces generated by DCs are smaller in magnitude than 
      found in neutrophils, and of similar magnitude during chemotaxis and 
      chemokinesis, at 18 +/- 1.4 and 16 +/- 1.3 nN/cell, respectively. The characteristic 
      duration of local DC traction forces was 3 min. The maximum principal stress in 
      the cell occurred in the plane perpendicular to the axis of motion, forward of 
      the centroid. We illustrate that the spatiotemporal pattern of traction stresses 
      can be used to predict the direction of future DC motion. Overall, DCs show a 
      mode of migration distinct from both mesenchymal cells and neutrophils, 
      characterized by rapid turnover of traction forces in leading filopodia.
CI  - Copyright (c) 2011 Biophysical Society. Published by Elsevier Inc. All rights 
      reserved.
FAU - Ricart, Brendon G
AU  - Ricart BG
AD  - Department of Chemical and Biomolecular Engineering, University of Pennsylvania, 
      Philadelphia, Pennsylvania, USA.
FAU - Yang, Michael T
AU  - Yang MT
FAU - Hunter, Christopher A
AU  - Hunter CA
FAU - Chen, Christopher S
AU  - Chen CS
FAU - Hammer, Daniel A
AU  - Hammer DA
LA  - eng
GR  - P41 EB001046/EB/NIBIB NIH HHS/United States
GR  - R01 AI082292/AI/NIAID NIH HHS/United States
GR  - AI082292/AI/NIAID NIH HHS/United States
GR  - EB001046/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Biophys J
JT  - Biophysical journal
JID - 0370626
RN  - 0 (Chemokine CCL19)
RN  - 9013-26-7 (Actomyosin)
SB  - IM
MH  - Actomyosin/metabolism
MH  - Animals
MH  - Biomechanical Phenomena/drug effects
MH  - *Cell Movement/drug effects
MH  - Chemokine CCL19/pharmacology
MH  - Dendritic Cells/*cytology/drug effects
MH  - Mice
MH  - Microfluidic Analytical Techniques/*instrumentation/*methods
MH  - Pseudopodia/drug effects/metabolism
MH  - *Stress, Mechanical
MH  - Surface Properties/drug effects
MH  - Time Factors
PMC - PMC3297797
EDAT- 2012/01/21 06:00
MHDA- 2012/05/10 06:00
PMCR- 2012/12/07
CRDT- 2012/01/21 06:00
PHST- 2011/07/15 00:00 [received]
PHST- 2011/09/06 00:00 [revised]
PHST- 2011/09/08 00:00 [accepted]
PHST- 2012/01/21 06:00 [entrez]
PHST- 2012/01/21 06:00 [pubmed]
PHST- 2012/05/10 06:00 [medline]
PHST- 2012/12/07 00:00 [pmc-release]
AID - S0006-3495(11)01082-4 [pii]
AID - BPJ3162 [pii]
AID - 10.1016/j.bpj.2011.09.022 [doi]
PST - ppublish
SO  - Biophys J. 2011 Dec 7;101(11):2620-8. doi: 10.1016/j.bpj.2011.09.022.