PMID- 22261109
OWN - NLM
STAT- MEDLINE
DCOM- 20120618
LR  - 20131121
IS  - 1873-3557 (Electronic)
IS  - 1386-1425 (Linking)
VI  - 89
DP  - 2012 Apr
TI  - Assessing the potential toxic effect of one persistent organic pollutant: 
      non-covalent interaction of dicofol with the enzyme trypsin.
PG  - 210-5
LID - 10.1016/j.saa.2011.12.070 [doi]
AB  - Because of the widespread concern that persistent organic pollutants (POPs) may 
      be adversely affecting the health of humans, reliable assessing their toxic 
      effects is urgently needed. We selectively study the interaction between dicofol 
      (DCF) and trypsin by steady state and time resolved fluorescence quenching 
      measurements and UV-visible absorption spectroscopy under physiological 
      conditions as well as applying molecular docking method to establish the 
      interaction model. The fluorescence results indicate DCF can spontaneously form a 
      complex with trypsin mainly by hydrogen bond with only one binding site, which 
      had been validated in molecular docking. The conformational change of trypsin was 
      proved by UV-visible absorption and synchronous fluorescence spectroscopy 
      indicating a red shift of carbonyl absorption peak. All the results indicated DCF 
      had potential toxic effects on both the structure and activity of the enzyme 
      trypsin and the effects enhanced with the increasing concentration of DCF.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Liu, Ying
AU  - Liu Y
AD  - Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of 
      Environmental Science and Engineering, America CRC for Environment & Health 
      Shandong Province, Shandong University, 27# Shanda South Road, Jinan 250100, PR 
      China.
FAU - Cao, Rongxiang
AU  - Cao R
FAU - Qin, Pengfei
AU  - Qin P
FAU - Liu, Rutao
AU  - Liu R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111231
PL  - England
TA  - Spectrochim Acta A Mol Biomol Spectrosc
JT  - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
JID - 9602533
RN  - 0 (Hazardous Substances)
RN  - 0 (Insecticides)
RN  - EC 3.4.21.4 (Trypsin)
RN  - W4WMM0WS91 (Dicofol)
SB  - IM
MH  - Binding Sites
MH  - Dicofol/chemistry/*metabolism/toxicity
MH  - Hazardous Substances/analysis/*metabolism/toxicity
MH  - Humans
MH  - Hydrogen Bonding
MH  - Insecticides/chemistry/*metabolism/toxicity
MH  - Models, Molecular
MH  - Protein Binding
MH  - Protein Conformation
MH  - Spectrometry, Fluorescence
MH  - Spectrophotometry, Ultraviolet
MH  - Trypsin/chemistry/*metabolism
EDAT- 2012/01/21 06:00
MHDA- 2012/06/19 06:00
CRDT- 2012/01/21 06:00
PHST- 2011/11/11 00:00 [received]
PHST- 2011/12/24 00:00 [accepted]
PHST- 2012/01/21 06:00 [entrez]
PHST- 2012/01/21 06:00 [pubmed]
PHST- 2012/06/19 06:00 [medline]
AID - S1386-1425(11)01156-5 [pii]
AID - 10.1016/j.saa.2011.12.070 [doi]
PST - ppublish
SO  - Spectrochim Acta A Mol Biomol Spectrosc. 2012 Apr;89:210-5. doi: 
      10.1016/j.saa.2011.12.070. Epub 2011 Dec 31.