PMID- 22261453
OWN - NLM
STAT- MEDLINE
DCOM- 20120306
LR  - 20191210
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 137
IP  - 2
DP  - 2012 Feb
TI  - Image analysis of HER2 immunohistochemical staining. Reproducibility and 
      concordance with fluorescence in situ hybridization of a laboratory-validated 
      scoring technique.
PG  - 270-6
LID - 10.1309/AJCP9MKNLHQNK2ZX [doi]
AB  - Image analysis of the HER2 immunohistochemical (IHC) stain can help determine 
      which breast cancer patients may benefit from HER2-targeted therapy. We studied 
      the concordance of HER2 IHC and fluorescence in situ hybridization (FISH) as well 
      as reproducibility of surgical pathologist (SP) and cytotechnologist (CT) 
      interpretations using manual and image analysis methodologies on 154 IHC cases. 
      Concordances with FISH were good for IHC negative (0, 1+) cases (range, 97%-100%) 
      and positive (3+) cases (range, 87%-100%). Image analysis had fewer equivocal 
      (2+) results (10.4%) than CT (14.9%) and SP (16.2%) manual methods, with higher 
      concordances to FISH (31%, 26%, and 20% for image analysis, CT manual, and SP 
      manual, respectively). CT manual (kappa = 0.747) and image analysis (kappa = 0.779) 
      methods had better interobserver reproducibility than SP manual (kappa = 0.697). CT 
      image analysis had better intraobserver reproducibility (kappa = 0.882) than CT (kappa = 
      0.828) and SP (kappa = 0.766) manual methods. HER2 IHC analysis performed by image 
      analysis can produce accurate results with improved reproducibility.
FAU - Minot, Douglas M
AU  - Minot DM
AD  - Dept. of Laboratory Medicine and Pathology, Mayo Clinic, 200 First St SW, 
      Rochester, MN 55905, USA.
FAU - Voss, Jesse
AU  - Voss J
FAU - Rademacher, Susan
AU  - Rademacher S
FAU - Lwin, Toe
AU  - Lwin T
FAU - Orsulak, Jessica
AU  - Orsulak J
FAU - Caron, Bolette
AU  - Caron B
FAU - Ketterling, Rhett
AU  - Ketterling R
FAU - Nassar, Aziza
AU  - Nassar A
FAU - Chen, Beiyun
AU  - Chen B
FAU - Clayton, Amy
AU  - Clayton A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Validation Study
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Antibodies, Monoclonal, Humanized)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (DNA, Neoplasm)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
RN  - P188ANX8CK (Trastuzumab)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antibodies, Monoclonal, Humanized/therapeutic use
MH  - Antineoplastic Agents/therapeutic use
MH  - Breast Neoplasms/*diagnosis/drug therapy/genetics/metabolism
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Humans
MH  - Image Processing, Computer-Assisted/*methods/standards
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Observer Variation
MH  - Receptor, ErbB-2/*genetics/*metabolism
MH  - Reproducibility of Results
MH  - Retrospective Studies
MH  - Trastuzumab
MH  - Young Adult
EDAT- 2012/01/21 06:00
MHDA- 2012/03/07 06:00
CRDT- 2012/01/21 06:00
PHST- 2012/01/21 06:00 [entrez]
PHST- 2012/01/21 06:00 [pubmed]
PHST- 2012/03/07 06:00 [medline]
AID - 137/2/270 [pii]
AID - 10.1309/AJCP9MKNLHQNK2ZX [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2012 Feb;137(2):270-6. doi: 10.1309/AJCP9MKNLHQNK2ZX.