PMID- 22262562
OWN - NLM
STAT- MEDLINE
DCOM- 20120723
LR  - 20120229
IS  - 1096-0929 (Electronic)
IS  - 1096-0929 (Linking)
VI  - 126
IP  - 1
DP  - 2012 Mar
TI  - Transcriptomic analysis of neurulation and early organogenesis in rat embryos: an 
      in vivo and ex vivo comparison.
PG  - 255-66
LID - 10.1093/toxsci/kfr343 [doi]
AB  - Cultured embryos mimic the morphological developmental progression of embryos (in 
      vivo) undergoing neurulation and early organogenesis. Using available genomics 
      technologies, comparative molecular-based assessments between cultured embryos 
      and in vivo models may further clarify commonalities and dissimilarities, which 
      contribute to differences between systems. Therefore, in this study, using a 
      transcriptomic approach, we compared cultured whole rat embryos and embryos in 
      vivo at comparable time points in development (gestational day (GD) 10 + 2-48 h, 
      GD 0 = copulatory plug) to assess for commonalities and differences in gene 
      expression in relation to morphology. We reveal strong parallels in 
      time-dependent expression of genes in terms of magnitude, directionality, and 
      functionality between whole embryo culture (WEC) and in vivo (rat). Genes 
      changing in expression over time resemble previously hypothesized mechanisms 
      underlying early development in mammalian systems. Furthermore, at the gene and 
      functional level, we identify genes, which differ in expression between models, 
      including genes related to development, oxygen transport, and metabolism. In 
      summary, our results support the use of WEC for toxicological studies aimed at 
      representing in vivo development during this time window at the molecular level. 
      Additionally, we indicate genes, which differ in expression between models, 
      providing possible insights for improvement of culture conditions.
FAU - Robinson, Joshua F
AU  - Robinson JF
AD  - Laboratory for Health Protection Research, National Institute for Public Health 
      and the Environment (RIVM), 3721 MA Bilthoven, the Netherlands.
FAU - Verhoef, Aart
AU  - Verhoef A
FAU - Piersma, Aldert H
AU  - Piersma AH
LA  - eng
SI  - GEO/GSE33195
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120118
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (RNA, Complementary)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - *Ectogenesis
MH  - Embryo Culture Techniques
MH  - Embryo, Mammalian/embryology/*metabolism
MH  - *Embryonic Development
MH  - Female
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation, Developmental
MH  - Models, Biological
MH  - *Neurulation
MH  - Oligonucleotide Array Sequence Analysis
MH  - *Organogenesis
MH  - Pregnancy
MH  - RNA/isolation & purification/metabolism
MH  - RNA, Complementary/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - *Transcriptome
EDAT- 2012/01/21 06:00
MHDA- 2012/07/24 06:00
CRDT- 2012/01/21 06:00
PHST- 2012/01/21 06:00 [entrez]
PHST- 2012/01/21 06:00 [pubmed]
PHST- 2012/07/24 06:00 [medline]
AID - kfr343 [pii]
AID - 10.1093/toxsci/kfr343 [doi]
PST - ppublish
SO  - Toxicol Sci. 2012 Mar;126(1):255-66. doi: 10.1093/toxsci/kfr343. Epub 2012 Jan 
      18.