PMID- 22263742
OWN - NLM
STAT- MEDLINE
DCOM- 20120716
LR  - 20201209
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 84
IP  - 4
DP  - 2012 Feb 21
TI  - Strong interaction between imidazolium-based polycationic polymer and 
      ferricyanide: toward redox potential regulation for selective in vivo 
      electrochemical measurements.
PG  - 1900-6
LID - 10.1021/ac202748s [doi]
AB  - This study effectively demonstrates a strategy to enable the ferricyanide-based 
      second-generation biosensors for selective in vivo measurements of 
      neurochemicals, with glucose as an example. The strategy is based on regulation 
      of redox potential of ferricyanide mediator by carefully controlling the 
      different adsorption ability of ferricyanide (Fe(CN)(6)(3-)) and ferrocyanide 
      (Fe(CN)(6)(4-)) onto electrode surface. To realize the negative shift of the 
      redox potential of Fe(CN)(6)(3-/4-), imidazolium-based polymer (Pim) is 
      synthesized and used as a matrix for surface adsorption of Fe(CN)(6)(3-/4-) due 
      to its stronger interaction with Fe(CN)(6)(3-) than with Fe(CN)(6)(4-). The 
      different adsorption ability of Fe(CN)(6)(3-) and Fe(CN)(6)(4-) onto electrodes 
      modified with a composite of Pim and multiwalled carbon nanotubes (MWNTs) 
      eventually enables the stable surface adsorption of both species to generate 
      integrated biosensors and, more importantly, leads to a negative shift of the 
      redox potential of the surface-confined redox mediator. Using glucose oxidase 
      (GOD) as the model biorecognition units, we demonstrate the validity of the 
      ferricyanide-based second-generation biosensors for selective in vivo 
      neurochemical measurements. We find that the biosensors developed with the 
      strategy demonstrated in this study can be used well as the selective detector 
      for continuous online detection of striatum glucose of guinea pigs, by 
      integration with in vivo microdialysis. This study essentially paves a new avenue 
      to developing electrochemical biosensors effectively for in vivo neurochemical 
      measurements, which is envisaged to be of great importance in understanding the 
      molecular basis of physiological and pathological events.
FAU - Zhuang, Xuming
AU  - Zhuang X
AD  - School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, 
      China.
FAU - Wang, Dalei
AU  - Wang D
FAU - Lin, Yuqing
AU  - Lin Y
FAU - Yang, Lifen
AU  - Yang L
FAU - Yu, Ping
AU  - Yu P
FAU - Jiang, Wei
AU  - Jiang W
FAU - Mao, Lanqun
AU  - Mao L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120209
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Ferricyanides)
RN  - 0 (Imidazoles)
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Polyamines)
RN  - 0 (Polyelectrolytes)
RN  - 0 (Polymers)
RN  - 0 (polycations)
RN  - 7GBN705NH1 (imidazole)
RN  - EC 1.1.3.4 (Glucose Oxidase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Basal Ganglia/metabolism
MH  - *Biosensing Techniques
MH  - *Electrochemical Techniques
MH  - Electrodes
MH  - Ferricyanides/*chemistry
MH  - Glucose/metabolism
MH  - Glucose Oxidase/metabolism
MH  - Guinea Pigs
MH  - Imidazoles/*chemistry
MH  - *Luminescent Measurements
MH  - Male
MH  - Nanotubes, Carbon
MH  - Oxidation-Reduction
MH  - Polyamines/*chemistry
MH  - Polyelectrolytes
MH  - Polymers/*chemistry
EDAT- 2012/01/24 06:00
MHDA- 2012/07/17 06:00
CRDT- 2012/01/24 06:00
PHST- 2012/01/24 06:00 [entrez]
PHST- 2012/01/24 06:00 [pubmed]
PHST- 2012/07/17 06:00 [medline]
AID - 10.1021/ac202748s [doi]
PST - ppublish
SO  - Anal Chem. 2012 Feb 21;84(4):1900-6. doi: 10.1021/ac202748s. Epub 2012 Feb 9.