PMID- 22264433 OWN - NLM STAT- MEDLINE DCOM- 20120618 LR - 20131121 IS - 1873-2976 (Electronic) IS - 0960-8524 (Linking) VI - 108 DP - 2012 Mar TI - Optimization of survival and spore formation of Paenibacillus polymyxa SQR-21 during bioorganic fertilizer storage. PG - 190-5 LID - 10.1016/j.biortech.2011.12.137 [doi] AB - The effects of storage temperature (20, 30 and 40 degrees C), inoculum type (pure spores, an equal mix of spores and vegetative cells and pure vegetative cells) and water content (20%, 30% and 40%) on the survival and spore formation of the biocontrol agent, Paenibacillus polymyxa SQR-21, in a bioorganic fertilizer were modeled in a 3x3x3 factorial design. Bacterial and spore populations were monitored by plate count and fluorescence in situ hybridization (FISH). Temperature significantly affected survival of inoculants after storage for 60 days. Populations were 1.48 (plate counting) or 1.71 (FISH) times greater when stored at 20 degrees C compared to 40 degrees C. Inoculation of the fertilizer with pure spores led to the highest spore formation percentage (67.6% for plate counting, 94.2% for FISH). This study provides useful information for preservation of bioorganic fertilizer. CI - Copyright (c) 2012 Elsevier Ltd. All rights reserved. FAU - Huo, Zhenhua AU - Huo Z AD - Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China. FAU - Zhang, Nan AU - Zhang N FAU - Xu, Zhihui AU - Xu Z FAU - Li, Shuqing AU - Li S FAU - Zhang, Qiuxia AU - Zhang Q FAU - Qiu, Meihua AU - Qiu M FAU - Yong, Xiaoyu AU - Yong X FAU - Huang, Qiwei AU - Huang Q FAU - Zhang, Ruifu AU - Zhang R FAU - Shen, Qi-rong AU - Shen QR LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20120104 PL - England TA - Bioresour Technol JT - Bioresource technology JID - 9889523 RN - 0 (Biological Control Agents) RN - 0 (Fertilizers) RN - 059QF0KO0R (Water) SB - IM MH - *Biological Control Agents MH - Fertilizers/*microbiology MH - In Situ Hybridization, Fluorescence MH - Paenibacillus/*physiology MH - Preservation, Biological/*methods MH - Spores, Bacterial/*growth & development MH - Survival Analysis MH - Temperature MH - Water/analysis EDAT- 2012/01/24 06:00 MHDA- 2012/06/19 06:00 CRDT- 2012/01/24 06:00 PHST- 2011/08/01 00:00 [received] PHST- 2011/11/29 00:00 [revised] PHST- 2011/12/27 00:00 [accepted] PHST- 2012/01/24 06:00 [entrez] PHST- 2012/01/24 06:00 [pubmed] PHST- 2012/06/19 06:00 [medline] AID - S0960-8524(11)01918-3 [pii] AID - 10.1016/j.biortech.2011.12.137 [doi] PST - ppublish SO - Bioresour Technol. 2012 Mar;108:190-5. doi: 10.1016/j.biortech.2011.12.137. Epub 2012 Jan 4.