PMID- 22264526
OWN - NLM
STAT- MEDLINE
DCOM- 20120815
LR  - 20120208
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 33
IP  - 11
DP  - 2012 Apr
TI  - BDNF blended chitosan scaffolds for human umbilical cord MSC transplants in 
      traumatic brain injury therapy.
PG  - 3119-26
LID - 10.1016/j.biomaterials.2012.01.009 [doi]
AB  - This study tested the cytotoxicity of a BDNF blended chitosan scaffold with human 
      umbilical cord mesenchymal stem cells (hUC-MSCs), and the in vitro effect of BDNF 
      blended chitosan scaffolds on neural stem cell differentiation with the aim of 
      contributing alternative methods in tissue engineering for the treatment of 
      traumatic brain injury (TBI). The chitosan scaffold based on immobilization of 
      BDNF by genipin (GP) as a crosslinking agent referred to hereafter as a CGB 
      scaffold was prepared by freezing-drying technique. hUC-MSCs were co-cultured 
      with the CGB scaffold. Fluorescent nuclear staining (Hoechst 33342) was employed 
      to determine the attachment of the hUC-MSCs to CGB scaffolds on the 1st, 3rd, 7th 
      and 10th day of co-culture. The viability of hUC-MSCs adhered to the CGB scaffold 
      was determined by digesting with 0.25% trypsin and evaluating with the cell 
      counting kit-8 (CCK-8). Prior to this, the diameter and porosity of CGB scaffolds 
      were measured. The amount of BDNF released from CGB over a 30 day period was 
      determined by ELISA. Finally, we investigated whether the released BDNF can 
      induce NSC to differentiate into neurons. There were no significant differences 
      in diameter and porosity of individual CGB scaffolds (P > 0.05). There were on 
      average more cells on the CGB scaffold on the first day than on any other day 
      sampled (P < 0.05). The CGB scaffolds released BDNF in a uniform profile, whereas 
      the CB scaffolds only released BDNF during the first 3 days. BDNF released from 
      CGB scaffold promoted neuronal differentiation of NSCs and led to significant 
      differences in differentiation rate and average neuron perimeter compared with 
      the control group. The results of this study demonstrate that CGB scaffolds are 
      biocompatible with hUC-MSCs and that granular CGB scaffolds covered with hUC-MSCs 
      are expected to generate new advances for future treatment of traumatic brain 
      injury.
CI  - Crown Copyright (c) 2012. Published by Elsevier Ltd. All rights reserved.
FAU - Shi, Wei
AU  - Shi W
AD  - Department of Neurosurgery, Surgical Comprehensive Laboratory, Affiliated 
      Hospital of Nantong University, Nantong 226001, PR China.
FAU - Nie, Dekang
AU  - Nie D
FAU - Jin, Guohua
AU  - Jin G
FAU - Chen, Weiwei
AU  - Chen W
FAU - Xia, Liang
AU  - Xia L
FAU - Wu, Xiujie
AU  - Wu X
FAU - Su, Xing
AU  - Su X
FAU - Xu, Xide
AU  - Xu X
FAU - Ni, Lanchun
AU  - Ni L
FAU - Zhang, Xianan
AU  - Zhang X
FAU - Zhang, Xinhua
AU  - Zhang X
FAU - Chen, Jian
AU  - Chen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120120
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Delayed-Action Preparations)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Animals
MH  - Brain Injuries/pathology/*surgery
MH  - Brain-Derived Neurotrophic Factor/*administration & dosage/chemistry
MH  - Cells, Cultured
MH  - Chitosan/*chemistry
MH  - Cord Blood Stem Cell Transplantation/*instrumentation
MH  - Delayed-Action Preparations/*administration & dosage/chemistry
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Mesenchymal Stem Cell Transplantation/*instrumentation
MH  - Neurons/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Tissue Scaffolds
EDAT- 2012/01/24 06:00
MHDA- 2012/08/16 06:00
CRDT- 2012/01/24 06:00
PHST- 2011/12/22 00:00 [received]
PHST- 2012/01/04 00:00 [accepted]
PHST- 2012/01/24 06:00 [entrez]
PHST- 2012/01/24 06:00 [pubmed]
PHST- 2012/08/16 06:00 [medline]
AID - S0142-9612(12)00013-0 [pii]
AID - 10.1016/j.biomaterials.2012.01.009 [doi]
PST - ppublish
SO  - Biomaterials. 2012 Apr;33(11):3119-26. doi: 10.1016/j.biomaterials.2012.01.009. 
      Epub 2012 Jan 20.