PMID- 22264717
OWN - NLM
STAT- MEDLINE
DCOM- 20120917
LR  - 20220623
IS  - 0015-5500 (Print)
IS  - 0015-5500 (Linking)
VI  - 57
IP  - 6
DP  - 2011
TI  - Role of transplanted bone marrow cells in response to skeletal muscle injury.
PG  - 232-41
AB  - The recently discovered capacity of bone marrow cells (BMCs) to contribute to 
      injury-induced skeletal muscle regeneration has brought new possibilities in the 
      treatment of skeletal muscle diseases. However, a suitable method of BMC 
      transplantation usable for such therapy has to be established. In this work, 
      recipient mice were intramuscularly injected with cardiotoxin, then whole-body 
      lethally irradiated to eradicate satellite cells in their injured tibialis 
      anterior (TA) muscles and to suppress haematopoiesis, and subsequently 
      intravenously transplanted with lacZ+ BMCs with the aim to investigate the role 
      of exogenous BMCs in response to skeletal muscle injury. Seven to 33 days after 
      grafting, recipient TA muscles were examined to detect donor-derived X-gal+ cells 
      and analysed by quantitative PCR. In injured recipients' muscles, X-gal 
      positivity was identified 14 and 33 days after grafting in some infiltrating 
      neutrophils and macrophages, infrequently in fibroblasts of endomysium, and in 
      many large multinucleated cells (devoid of myogenic markers desmin and nestin) 
      resembling foreign body giant cells situated in the vicinity of necrotic muscle 
      fibres. qPCR confirmed the presence of transplanted lacZ+ BMCs in injured 
      recipients' muscles. Our results proved the ability of intravenously transplanted 
      adult BMCs to settle in injured muscles and generate blood cells that infiltrated 
      endomysium and took part in the cleaning reaction. After inhibition of endogenous 
      myogenesis, BMCs were not able to participate in formation of new muscle fibres 
      due to persisting necrosis of degenerated muscle fibres. Instead, BMCs attempted 
      to resorb necrotic structures, which confirmed the indispensable role of bone 
      marrow-derived macrophages in skeletal muscle regeneration.
FAU - Cizkova, D
AU  - Cizkova D
AD  - Department of Histology and Embryology, Faculty of Medicine in Hradec Kralove, 
      Charles University in Prague, Czech Republic. cizkovad@lfhk.cuni.cz
FAU - Vavrova, J
AU  - Vavrova J
FAU - Micuda, S
AU  - Micuda S
FAU - Filip, S
AU  - Filip S
FAU - Brcakova, E
AU  - Brcakova E
FAU - Bruckova, L
AU  - Bruckova L
FAU - Mokry, J
AU  - Mokry J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Czech Republic
TA  - Folia Biol (Praha)
JT  - Folia biologica
JID - 0234640
RN  - 9007-49-2 (DNA)
RN  - EC 3.2.1.31 (Glucuronidase)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - *Bone Marrow Transplantation
MH  - DNA/metabolism
MH  - Glucuronidase/metabolism
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscle, Skeletal/*injuries/*pathology
MH  - Whole-Body Irradiation
EDAT- 2012/01/24 06:00
MHDA- 2012/09/18 06:00
CRDT- 2012/01/24 06:00
PHST- 2012/01/24 06:00 [entrez]
PHST- 2012/01/24 06:00 [pubmed]
PHST- 2012/09/18 06:00 [medline]
AID - file/5604/FB2011A0033.pdf [pii]
PST - ppublish
SO  - Folia Biol (Praha). 2011;57(6):232-41.