PMID- 22266352
OWN - NLM
STAT- MEDLINE
DCOM- 20121011
LR  - 20220410
IS  - 1878-1519 (Electronic)
IS  - 1569-9048 (Linking)
VI  - 182
IP  - 1
DP  - 2012 Jun 15
TI  - Protective effects of bone marrow mononuclear cell therapy on lung and heart in 
      an elastase-induced emphysema model.
PG  - 26-36
LID - 10.1016/j.resp.2012.01.002 [doi]
AB  - We hypothesized that bone marrow-derived mononuclear cell (BMDMC) therapy 
      protects the lung and consequently the heart in experimental elastase-induced 
      emphysema. Twenty-four female C57BL/6 mice were intratracheally instilled with 
      saline (C group) or porcine pancreatic elastase (E group) once a week during 4 
      weeks. C and E groups were randomized into subgroups receiving saline (SAL) or 
      male BMDMCs (2 x 10(6), CELL) intravenously 3h after the first saline or elastase 
      instillation. Compared to E-SAL group, E-CELL mice showed, at 5 weeks: lower mean 
      linear intercept, neutrophil infiltration, elastolysis, collagen fiber deposition 
      in alveolar septa and pulmonary vessel wall, lung cell apoptosis, right ventricle 
      wall thickness and area, higher endothelial growth factor and insulin-like growth 
      factor mRNA expressions in lung tissue, and reduced platelet-derived growth 
      factor, transforming growth factor-beta, and caspase-3 expressions. In conclusion, 
      BMDMC therapy was effective at modulating the inflammatory and remodeling 
      processes in the present model of elastase-induced emphysema.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Cruz, Fernanda F
AU  - Cruz FF
AD  - Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of 
      Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
FAU - Antunes, Mariana A
AU  - Antunes MA
FAU - Abreu, Soraia C
AU  - Abreu SC
FAU - Fujisaki, Livia C
AU  - Fujisaki LC
FAU - Silva, Johnatas D
AU  - Silva JD
FAU - Xisto, Debora G
AU  - Xisto DG
FAU - Maron-Gutierrez, Tatiana
AU  - Maron-Gutierrez T
FAU - Ornellas, Debora S
AU  - Ornellas DS
FAU - Sa, Vanessa K
AU  - Sa VK
FAU - Rocha, Nazareth N
AU  - Rocha NN
FAU - Capelozzi, Vera L
AU  - Capelozzi VL
FAU - Morales, Marcelo M
AU  - Morales MM
FAU - Rocco, Patricia R M
AU  - Rocco PR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120113
PL  - Netherlands
TA  - Respir Physiol Neurobiol
JT  - Respiratory physiology & neurobiology
JID - 101140022
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - EC 3.4.21.36 (Pancreatic Elastase)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Airway Remodeling
MH  - Analysis of Variance
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Caspase 3/metabolism
MH  - Echocardiography
MH  - Emphysema/chemically induced/metabolism/pathology/*therapy
MH  - Female
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Leukocytes, Mononuclear/*transplantation
MH  - Lung/metabolism/*pathology
MH  - Lymphocyte Subsets/cytology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Myocardium/metabolism/pathology
MH  - Pancreatic Elastase
MH  - Pulmonary Heart Disease/*prevention & control
MH  - Random Allocation
EDAT- 2012/01/24 06:00
MHDA- 2012/10/12 06:00
CRDT- 2012/01/24 06:00
PHST- 2011/09/05 00:00 [received]
PHST- 2012/01/07 00:00 [revised]
PHST- 2012/01/08 00:00 [accepted]
PHST- 2012/01/24 06:00 [entrez]
PHST- 2012/01/24 06:00 [pubmed]
PHST- 2012/10/12 06:00 [medline]
AID - S1569-9048(12)00003-1 [pii]
AID - 10.1016/j.resp.2012.01.002 [doi]
PST - ppublish
SO  - Respir Physiol Neurobiol. 2012 Jun 15;182(1):26-36. doi: 
      10.1016/j.resp.2012.01.002. Epub 2012 Jan 13.