PMID- 2226740
OWN - NLM
STAT- MEDLINE
DCOM- 19901210
LR  - 20190907
IS  - 0902-4441 (Print)
IS  - 0902-4441 (Linking)
VI  - 45
IP  - 4
DP  - 1990 Oct
TI  - Labelling autologous platelets with 111In tropolonate for platelet kinetic 
      studies: limitations imposed by thrombocytopenia.
PG  - 223-30
AB  - An in vitro method of radiolabelling platelets with 111In tropolonate in plasma 
      has been devised enabling imaging and cell kinetic studies to be performed in 
      patients with thrombocytopenia (TP) using autologous, rather than donor, 
      platelets. Platelets from 10 TP patients, with platelet counts ranging from 4-91 
      x 10(9)/l, were labelled in 50% plasma with 111In tropolonate, containing the 
      optimum tropolone concentration of 2 x 10(-4) mol/l, at platelet concentrations 
      ranging from 0.08-4.5 x 10(9)/ml resulting in labelling efficiencies (LE) between 
      51 and 86%. In 4 patients, red cells contaminated the platelets but this was 
      corrected for by measuring the proportion of 111In on the platelets prior to 
      injection and in the post-injection blood samples. Platelet recovery (PR), mean 
      platelet life-span (MPLS) and platelet production rate (PPR) were calculated and 
      splenic and hepatic images were taken. The results clearly show that useful 
      clinical data can be obtained by this method even in patients with severe TP.
FAU - Danpure, H J
AU  - Danpure HJ
AD  - MRC Cyclotron Unit, Hammersmith Hospital, London, U.K.
FAU - Osman, S
AU  - Osman S
FAU - Peters, A M
AU  - Peters AM
LA  - eng
PT  - Journal Article
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
RN  - 0 (Indium Radioisotopes)
RN  - 0 (Organometallic Compounds)
RN  - 14283-79-5 (indium tris(tropolonate))
RN  - 7L6DL16P1T (Tropolone)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Blood Platelets/pathology/*physiology
MH  - Cell Separation
MH  - Cell Survival
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - *Indium Radioisotopes
MH  - Isotope Labeling/*methods
MH  - Kinetics
MH  - Liver/pathology
MH  - Male
MH  - Middle Aged
MH  - *Organometallic Compounds
MH  - Platelet Count
MH  - Spleen/pathology
MH  - Thrombocytopenia/*blood
MH  - Tropolone/*analogs & derivatives
EDAT- 1990/10/01 00:00
MHDA- 1990/10/01 00:01
CRDT- 1990/10/01 00:00
PHST- 1990/10/01 00:00 [pubmed]
PHST- 1990/10/01 00:01 [medline]
PHST- 1990/10/01 00:00 [entrez]
AID - 10.1111/j.1600-0609.1990.tb00461.x [doi]
PST - ppublish
SO  - Eur J Haematol. 1990 Oct;45(4):223-30. doi: 10.1111/j.1600-0609.1990.tb00461.x.