PMID- 22268355
OWN - NLM
STAT- MEDLINE
DCOM- 20121015
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 10
DP  - 2011 Oct
TI  - [Influences of ion-suppressors on retention behaviors of nine food additives in 
      reversed-phase high performance liquid chromatographic separation].
PG  - 988-94
AB  - The influences of ion-suppressors on retention behaviors of nine food additives, 
      i.e., acesulfame, saccharin, caffeine, aspartame, benzoic acid, sorbic acid, 
      stevioside, dehydroacetic acid and neotame in reversed-phase high performance 
      liquid chromatographic (RP-HPLC) separation were investigated. The organic 
      modification effects of acids, i. e. , trifluoroacetic acid (TFA) and buffer 
      salts, i. e. , TFA-ammonium acetate (AmAc) were studied emphatically. The 
      relationships between retention factors of solutes and volume percentages of 
      ion-suppressors in the mobile phase systems of acetonitrile-TFA aqueous solution 
      and acetonitrile-TFA-AmAc aqueous solution were quantitatively established, 
      separately. The separation of nine food additives was completed by a gradient 
      elution with acetonitrile-TFA (0.01%, v/v)-AmAc (2. 5 mmol/L) aqueous solution as 
      the mobile phases. An RP-HPLC method was established for the simultaneous 
      determination of nine food additives in red wine. In the range of 10. 0 - 100. 0 
      mg/L, nine food additives showed good linearity with the correlation coefficients 
      ( r2 ) larger than 0. 999 1. The limits of detection (LODs) were in the range of 
      0. 33 - 2. 36 mg/L and the limits of quantification (LOQs) were in the range of 
      1. 11 - 7. 80 mg/L. The spiked recoveries were between 87. 61% and 108. 4% with 
      the relative standard deviations (RSDs) of 2. 2% -9. 4%. These results are of 
      referential significance for the rapid establishment and accu- rate optimization 
      of RP-HPLC separation for the simultaneous determination of food additives in 
      other foods.
FAU - Zhao, Yonggang
AU  - Zhao Y
AD  - Ningbo Municipal Center for Disease Control and Prevention, Ningbo Key Laboratory 
      of Poison Research and Control, Ningbo 315010, China.
FAU - Chen, Xiaohong
AU  - Chen X
FAU - Li, Xiaoping
AU  - Li X
FAU - Yao, Shanshan
AU  - Yao S
FAU - Jin, Micong
AU  - Jin M
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Food Additives)
RN  - 0 (Ions)
RN  - 3G6A5W338E (Caffeine)
RN  - FST467XS7D (Saccharin)
RN  - Z0H242BBR1 (Aspartame)
SB  - IM
MH  - Aspartame/analysis
MH  - Caffeine/analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Chromatography, Reverse-Phase/*methods
MH  - Food Additives/*analysis
MH  - Ions/*antagonists & inhibitors
MH  - Saccharin/analysis
EDAT- 2012/01/25 06:00
MHDA- 2012/10/16 06:00
CRDT- 2012/01/25 06:00
PHST- 2012/01/25 06:00 [entrez]
PHST- 2012/01/25 06:00 [pubmed]
PHST- 2012/10/16 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Oct;29(10):988-94.