PMID- 22268357
OWN - NLM
STAT- MEDLINE
DCOM- 20121015
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 10
DP  - 2011 Oct
TI  - [Simultaneous determination of exogenous phosphocreatine and its metabolite 
      creatine in rabbit plasma using ion-pair reversed-phase high performance liquid 
      chromatography].
PG  - 1000-4
AB  - A method for simultaneous determination of exogenous phosphocreatine (PCr) and 
      its metabolite creatine (Cr) in rabbit plasma was developed by using an ion-pair 
      reversed-phase high performance liquid chromatography (IP-RP-HPLC). The 
      pharmacokinetics (PK) of PCr was also investigated. In the IP-RP-HPLC method, a 
      Kromasil C,8 column was used with methanol and phosphate buffer containing 
      tetrabutylammonium hydrogen sulfate (TBA, ion-pair reagent) as the mobile phases 
      in a gradient elution mode, while changing detection wavelength and flow rate. 
      The internal standard method was used to quantify PCr and Cr, and the baseline 
      subtraction method was applied. The calibration curves showed good linearity 
      ranged from 10 to 7 500 mg/L for PCr and from 10 to 1 500 mg/L for Cr, and the 
      correlation coefficients (r) were greater than 0. 999. The methodology validation 
      showed high specificity, precision and recovery with the intra-day and inter-day 
      relative standard deviations (RSDs) of not more than 6. 2%, accuracies of 96. 5% 
      - 102. 4%, and extraction recoveries of more than 92%. After intravenous 
      injection of PCr, the concentration-time profile can be best described by 
      two-compartment model with elimination half time of (20.4 +/- 2.7) min, apparent 
      volume of distribution of (0.179 +/- 0.037) L/kg and clearance rate of (0.019 +/- 
      0. 002) L/(kg x min). The Cr appeared rapidly with time to maximal concentration 
      of 30 min, elimination half time of (43.7 +/- 4. 5) min. The results of practical 
      application showed that this bio-analytical method can completely meet the 
      requirements for PK study of PCr in rabbit plasma.
FAU - Xi, Heng
AU  - Xi H
AD  - College of Pharmacy, Dalian Medical University, Dalian 116044, China.
FAU - Han, Guozhu
AU  - Han G
FAU - Lu, Li
AU  - Lu L
FAU - Zhang, Di
AU  - Zhang D
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 020IUV4N33 (Phosphocreatine)
RN  - MU72812GK0 (Creatine)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Chromatography, Reverse-Phase/*methods
MH  - Creatine/*blood
MH  - Female
MH  - Male
MH  - Phosphocreatine/*blood/pharmacokinetics
MH  - Rabbits
EDAT- 2012/01/25 06:00
MHDA- 2012/10/16 06:00
CRDT- 2012/01/25 06:00
PHST- 2012/01/25 06:00 [entrez]
PHST- 2012/01/25 06:00 [pubmed]
PHST- 2012/10/16 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Oct;29(10):1000-4.