PMID- 22268358
OWN - NLM
STAT- MEDLINE
DCOM- 20121015
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 10
DP  - 2011 Oct
TI  - [Simultaneous determination of ibuprofen and arginine in ibuprofen injection 
      using ultra performance liquid chromatography].
PG  - 1005-9
AB  - An ultra performance liquid chromatography-photodiode array detector (UPLC-PDA) 
      method was developed for the determination of ibuprofen and the solubilizer 
      arginine in ibuprofen injection. 2,4-Dinitroflurobenzene (DNFB) was used as the 
      precolumn derivatization reagent. The separation of ibuprofen and arginine 
      derivative was performed on a BEH C18 column (50 mm x 2. 1 mm, 1.7 microm) with 
      the mobile phase consisting of acetonitrile-0. 05 mol/L potassium dihydrogen 
      phosphate (pH 2. 5) in a gradient elution mode at a flow rate of 0.4 mL/min. 
      Ultraviolet absorption detection wavelengths were set at 357 nm for arginine 
      derivative and 220 nm for ibuprofen. The column temperature was set at 30 degrees 
      C. Good linearities were obtained in the ranges of 2.0 - 100.5 mg/L for ibuprofen 
      and 1.7 - 84.5 mg/L for arginine, both with the correlation coefficients (r) of 
      0. 999 7. The spiked recoveries were 99. 8% and 99. 6% with the relative standard 
      deviations (RSDs) of 0. 37% and 0. 25% for ibuprofen and arginine, respectively. 
      Their respective limits of quantification (LOQs) (S/N = 10) were 0. 1 ng and 0. 2 
      ng, and the limits of detection (LODs) (S/N= 3) were 0.03 ng and 0.05 ng. The 
      results demonstrated that the proposed method is simple, accurate, reproducible 
      and suitable for the quality control of ibuprofen injection.
FAU - Liu, Jing
AU  - Liu J
AD  - Shenyang Pharmaceutical University, Shenyang 110016, China.
FAU - Ma, Hequn
AU  - Ma H
FAU - Zhu, Meng
AU  - Zhu M
FAU - Wang, Huan
AU  - Wang H
FAU - Zhang, Tianhong
AU  - Zhang T
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 94ZLA3W45F (Arginine)
RN  - WK2XYI10QM (Ibuprofen)
SB  - IM
MH  - *Anti-Inflammatory Agents, Non-Steroidal/administration & dosage/analysis
MH  - Arginine/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Ibuprofen/administration & dosage/*analysis
MH  - Injections, Intravenous
MH  - Solubility
EDAT- 2012/01/25 06:00
MHDA- 2012/10/16 06:00
CRDT- 2012/01/25 06:00
PHST- 2012/01/25 06:00 [entrez]
PHST- 2012/01/25 06:00 [pubmed]
PHST- 2012/10/16 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2011 Oct;29(10):1005-9.