PMID- 22274315
OWN - NLM
STAT- MEDLINE
DCOM- 20120710
LR  - 20220330
IS  - 1525-1438 (Electronic)
IS  - 1048-891X (Linking)
VI  - 22
IP  - 2
DP  - 2012 Feb
TI  - Association of interleukin 16 with the development of ovarian tumor and 
      tumor-associated neoangiogenesis in laying hen model of spontaneous ovarian 
      cancer.
PG  - 199-207
LID - 10.1097/IGC.0b013e318236a27b [doi]
AB  - OBJECTIVE: Tumor-associated neoangiogenesis (TAN) is an early event in ovarian 
      tumor development. Interleukin 16 (IL-16) is a proangiogenic cytokine that 
      stimulates production of neoangiogenic factors. The goal of this study was to 
      determine the association of IL-16 with tumor development and ovarian TAN in 
      laying hens, an animal model of spontaneous ovarian cancer (OVCA). METHODS: Sera 
      and ovarian tissues from 3-year-old laying hens were collected and processed for 
      histopathologic, immunoassay, immunohistochemistry, immunoblotting, and molecular 
      biological studies to determine the tissue expression and serum levels of IL-16. 
      Samples were divided into 3 groups based on the diagnosis of the histopathologic 
      ovarian tissue examination, namely, normal (healthy control, n = 81), early (n = 
      23 including 11 with microscopic OVCA), and late stages (n = 16) of OVCA. 
      RESULTS: Serum levels of IL-16 were significantly higher in hens with 
      microscopic, early, and late stages of OVCA than normal hens (P < 0.0001). The 
      frequencies of IL-16 cells in tumor-bearing ovaries were significantly higher 
      than normal hens (P < 0.05). The expression of IL-16 protein and mRNA were 
      stronger in tumor-bearing ovaries than normal ovaries. In addition to ovarian 
      stroma, IL-16 was also expressed by the epithelial cells of the tumor in OVCA 
      hens. Differences in serum levels and ovarian IL-16 expression were not 
      significant among different histological subtypes of OVCA including serous, 
      endometrioid, and mucinous. Similar to the serum levels and ovarian expression of 
      IL-16, the densities of neoangiogenic microvessels were significantly higher in 
      hens with tumor-bearing ovaries than normal hens. CONCLUSIONS: The results of the 
      study suggest that changes in serum levels of IL-16 are associated with tumor 
      development and TAN. Thus, serum IL-16 levels may be an indicator of ovarian TAN 
      at the early stage of OVCA.
FAU - Yellapa, Aparna
AU  - Yellapa A
AD  - Department of Pharmacology, Rush University Medical Center, Chicago, IL, USA.
FAU - Bahr, Janice M
AU  - Bahr JM
FAU - Bitterman, Pincas
AU  - Bitterman P
FAU - Abramowicz, Jacques S
AU  - Abramowicz JS
FAU - Edassery, Seby L
AU  - Edassery SL
FAU - Penumatsa, Krishna
AU  - Penumatsa K
FAU - Basu, Sanjib
AU  - Basu S
FAU - Rotmensch, Jacob
AU  - Rotmensch J
FAU - Barua, Animesh
AU  - Barua A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Gynecol Cancer
JT  - International journal of gynecological cancer : official journal of the 
      International Gynecological Cancer Society
JID - 9111626
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Interleukin-16)
SB  - IM
MH  - Animals
MH  - Biomarkers, Tumor/*blood
MH  - Chickens
MH  - Disease Models, Animal
MH  - Female
MH  - Interleukin-16/*blood
MH  - Neoplasm Staging
MH  - Neovascularization, Pathologic/blood/diagnosis/pathology
MH  - Ovarian Neoplasms/blood/*diagnosis/pathology
EDAT- 2012/01/26 06:00
MHDA- 2012/07/11 06:00
CRDT- 2012/01/26 06:00
PHST- 2012/01/26 06:00 [entrez]
PHST- 2012/01/26 06:00 [pubmed]
PHST- 2012/07/11 06:00 [medline]
AID - 00009577-201202000-00008 [pii]
AID - 10.1097/IGC.0b013e318236a27b [doi]
PST - ppublish
SO  - Int J Gynecol Cancer. 2012 Feb;22(2):199-207. doi: 10.1097/IGC.0b013e318236a27b.