PMID- 22275807
OWN - NLM
STAT- MEDLINE
DCOM- 20120910
LR  - 20211021
IS  - 1090-0535 (Electronic)
IS  - 1090-0535 (Linking)
VI  - 18
DP  - 2012
TI  - Comparison of beneficial factors for corneal wound-healing of rat mesenchymal 
      stem cells and corneal limbal stem cells on the xenogeneic acellular corneal 
      matrix in vitro.
PG  - 161-73
AB  - PURPOSE: This experiment aims to investigate the potential ability of mesenchymal 
      stem cells (MSCs) to produce beneficial factors for corneal recovery when the 
      cells were seeded on a xenogeneic acellular corneal matrix (ACM) in vitro. 
      METHODS: MSCs and corneal limbal stem cells (LSCs) from the corneal limbus region 
      of rats were isolated and cultured. Batch 1 from each type of cell was seeded on 
      a Beagle cornea ACM at a density of 3x10(3) cells/mm(2) for 7 days. The 
      proliferation activity of the cells was quantitatively determined at 1, 3, 5, and 
      7 days with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) 
      assay. Keratin3/12 (CK3/12) growth factors, including vascular endothelial growth 
      factor (VEGF), pigment epithelium-derived factor, epidermal growth factor, and 
      transforming growth factor-beta1, integrin subunits such as alpha(5)beta(1), and 
      alpha(6)beta(1), and elements of the extracellular matrix (ECM) such as fibronectin and 
      laminin were investigated with immunofluorescence staining, reverse 
      transcriptional polymerase chain reaction, and western blot assay, respectively. 
      RESULTS: The basic expression of growth factors in MSCs was much higher (n=6, 
      p<0.05) than that in the LSCs, including VEGF, epidermal growth factor, and 
      transforming growth factor-beta1. After being seeded on the ACM, those factors in 
      MSCs expressed continuously at a high level, but the seeded corneal epithelium 
      cells presented a downregulated trend in these factors. The expression of VEGF in 
      seeded MSCs decreased, which was similar to the trend for the seeded LSCs (n=6, 
      p<0.05). The expression of keratin3, a sign of mature epithelium cells, was also 
      present in the MSCs after being seeded for 7 days. The expression of pigment 
      epithelium-derived factor by the seeded and normal culture MSCs was equal, while 
      the expression of this factor was not detected in either the seeded or the normal 
      cultured LSCs. There were no significant differences between the integrin 
      subunits (alpha5, alpha6, beta1) and the extracellular matrix, including fibronectin and 
      laminin, generated by normal cultured or seeded MSCs and LSCs. CONCLUSIONS: Under 
      the ACM microenvironment, the MSCs presented beneficial factors for corneal 
      recovery comparable to those presented by corneal LSCs. This indicates that MSCs, 
      when combined with an ACM, may compose a competent corneal substitute for healing 
      corneal wounds.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Department of Ophthalmology, Peking University Third Hospital, Beijing, China.
FAU - Huang, Chen
AU  - Huang C
FAU - Feng, Yun
AU  - Feng Y
FAU - Li, Ying
AU  - Li Y
FAU - Wang, Wei
AU  - Wang W
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120120
PL  - United States
TA  - Mol Vis
JT  - Molecular vision
JID - 9605351
RN  - 0 (Integrins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (RNA, Messenger)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Differentiation
MH  - Cell Shape
MH  - Cells, Cultured
MH  - Cornea/metabolism/*pathology
MH  - Dogs
MH  - Extracellular Matrix/*metabolism
MH  - Fluorescent Antibody Technique
MH  - Integrins/genetics/metabolism
MH  - Intercellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Keratins/genetics/metabolism
MH  - Limbus Corneae/*cytology
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Wound Healing
PMC - PMC3265173
EDAT- 2012/01/26 06:00
MHDA- 2012/09/11 06:00
PMCR- 2012/01/01
CRDT- 2012/01/26 06:00
PHST- 2011/06/13 00:00 [received]
PHST- 2012/01/16 00:00 [accepted]
PHST- 2012/01/26 06:00 [entrez]
PHST- 2012/01/26 06:00 [pubmed]
PHST- 2012/09/11 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - 19 [pii]
AID - 2011MOLVIS0264 [pii]
PST - ppublish
SO  - Mol Vis. 2012;18:161-73. Epub 2012 Jan 20.