PMID- 22278837
OWN - NLM
STAT- MEDLINE
DCOM- 20120710
LR  - 20211021
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 50
IP  - 4
DP  - 2012 Apr
TI  - Development of a human herpesvirus 6 species-specific immunoblotting assay.
PG  - 1245-51
LID - 10.1128/JCM.05834-11 [doi]
AB  - In order to assess the full spectrum of human herpesvirus 6A (HHV-6A)- and 
      HHV-6B-associated diseases, we sought to develop an HHV-6 species-specific 
      serological assay based on immunoblot analysis. The immunodominant proteins 
      encoded by open reading frame U11, p100 for HHV-6A (strain U1102) and 101K for 
      HHV-6B (strain Z29), were selected to generate virus species-specific antigens. 
      Recombinant p100 and 101K were produced in a prokaryotic expression system. The 
      expression of these proteins was confirmed by using anti-His tag and 
      101K-specific monoclonal antibodies. HHV-6 species-specific antibodies were 
      detected by immunoblotting in patient sera. Eighty-seven serum samples obtained 
      from various subjects were utilized to determine the reliability of the method 
      for clinical use. Ten of twelve exanthem subitum convalescent-phase sera reacted 
      exclusively with 101K, whereas none of twelve acute-phase sera reacted with 
      either protein. Two of three sera collected from HHV-6A-infected patients reacted 
      with p100 and 101K. Although all five acute and convalescent-phase sera obtained 
      from transplant recipients reacted exclusively with 101K, two of six 
      convalescent-phase sera obtained from patients with drug-induced hypersensitivity 
      syndrome reacted with both p100 and 101K. Of 38 sera obtained from healthy 
      adults, 31 were positive for 101K antibody, while 4 reacted with both proteins. 
      However, PCR analysis of peripheral blood mononuclear cells and saliva from these 
      subjects did not detect HHV-6A DNA. In conclusion, this novel serological assay 
      based on immunoblot analysis using recombinant HHV-6A p100 and HHV-6B 101K 
      allowed us to discriminate between HHV-6A- and HHV-6B-specific antibodies.
FAU - Higashimoto, Yuki
AU  - Higashimoto Y
AD  - Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, 
      Aichi, Japan.
FAU - Ohta, Akane
AU  - Ohta A
FAU - Nishiyama, Yukihiro
AU  - Nishiyama Y
FAU - Ihira, Masaru
AU  - Ihira M
FAU - Sugata, Ken
AU  - Sugata K
FAU - Asano, Yoshizo
AU  - Asano Y
FAU - Peterson, Daniel L
AU  - Peterson DL
FAU - Ablashi, Dharam V
AU  - Ablashi DV
FAU - Lusso, Paolo
AU  - Lusso P
FAU - Yoshikawa, Tetsushi
AU  - Yoshikawa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120125
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antibodies, Viral/*blood
MH  - Antigens, Viral/*immunology
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Child
MH  - Child, Preschool
MH  - DNA, Viral/blood
MH  - Exanthema Subitum/blood/*diagnosis/immunology/virology
MH  - Female
MH  - Herpesvirus 6, Human/*immunology
MH  - Humans
MH  - Infant
MH  - Leukocytes, Mononuclear/virology
MH  - Male
MH  - Middle Aged
MH  - Young Adult
PMC - PMC3318559
EDAT- 2012/01/27 06:00
MHDA- 2012/07/11 06:00
PMCR- 2012/10/01
CRDT- 2012/01/27 06:00
PHST- 2012/01/27 06:00 [entrez]
PHST- 2012/01/27 06:00 [pubmed]
PHST- 2012/07/11 06:00 [medline]
PHST- 2012/10/01 00:00 [pmc-release]
AID - JCM.05834-11 [pii]
AID - 05834-11 [pii]
AID - 10.1128/JCM.05834-11 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2012 Apr;50(4):1245-51. doi: 10.1128/JCM.05834-11. Epub 2012 
      Jan 25.