PMID- 22279590
OWN - NLM
STAT- MEDLINE
DCOM- 20120709
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 1
DP  - 2012
TI  - Capacities of migrating CD1b+ lymph dendritic cells to present Salmonella 
      antigens to naive T cells.
PG  - e30430
LID - 10.1371/journal.pone.0030430 [doi]
LID - e30430
AB  - Dendritic cells (DCs) are well known as professional antigen-presenting cells 
      (APC) able to initiate specific T-cell responses to pathogens in lymph nodes (LN) 
      draining the site of infection. However, the respective contribution of migratory 
      and LN-resident DCs in this process remains unclear. As DC subsets represent 
      important targets for vaccination strategies, more precise knowledge of DC 
      subsets able to present vaccine antigens to T cells efficiently is required. To 
      investigate the capacities of DCs migrating in the lymph (L-DCs) to initiate a 
      specific T-cell response, we used physiologically generated DCs collected from a 
      pseudoafferent lymphatic cannulation model in sheep. The CD1b+ L-DCs were 
      assessed for presenting antigens from the vaccine attenuated strain of Salmonella 
      enterica serovar Abortusovis. CD1b+ L-DCs were able to phagocytose, process and 
      to present efficiently Salmonella antigens to effector/memory T cells in vitro. 
      They were shown to be efficient APC for the priming of allogeneic naive T cells 
      associated with inducing both IFN-gamma and IL-4 responses. They were also efficient 
      in presenting Salmonella antigens to autologous naive T cells associated with 
      inducing both IFN-gamma and IL-10 responses. The capacities of L-DCs to process and 
      present Salmonella antigens to T cells were investigated in vivo after 
      conjunctival inoculation of Salmonella. The CD1b+ L-DCs collected after 
      inoculation were able to induce the proliferative response of CD4+ T cells 
      suggesting the in vivo capture of Salmonella antigens by the CD1b+ L-DCs, and 
      their potential to present them directly to CD4+ T cells. In this study, CD1b+ 
      L-DCs present potential characteristics of APC to initiate by themselves T cell 
      priming in the LN. They could be used as target cells for driving immune 
      activation in vaccinal strategies.
FAU - Olivier, Michel
AU  - Olivier M
AD  - UR1282 Infectiologie Animale et Sante Publique, Institut National de la Recherche 
      Agronomique, Nouzilly, France.
FAU - Foret, Benjamin
AU  - Foret B
FAU - Le Vern, Yves
AU  - Le Vern Y
FAU - Guilloteau, Laurence A
AU  - Guilloteau LA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120118
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1b antigen)
RN  - 126880-86-2 (L-Selectin)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Antigens, Bacterial/*immunology
MH  - Antigens, CD1/immunology/metabolism
MH  - CD4-Positive T-Lymphocytes/immunology/metabolism
MH  - Cell Movement/immunology
MH  - Cells, Cultured
MH  - Conjunctiva/immunology/microbiology
MH  - Dendritic Cells/*immunology/metabolism
MH  - Endocytosis/immunology
MH  - Female
MH  - Flow Cytometry
MH  - Immunophenotyping
MH  - Interferon-gamma/immunology/metabolism
MH  - Interleukin-4/immunology/metabolism
MH  - L-Selectin/immunology/metabolism
MH  - Lymph/cytology/immunology
MH  - Lymph Nodes/cytology/immunology
MH  - Phagocytosis/immunology
MH  - Salmonella Infections/immunology/microbiology
MH  - Salmonella enterica/*immunology
MH  - Sheep
MH  - T-Lymphocytes/*immunology/metabolism
PMC - PMC3261196
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/01/27 06:00
MHDA- 2012/07/10 06:00
PMCR- 2012/01/18
CRDT- 2012/01/27 06:00
PHST- 2011/06/15 00:00 [received]
PHST- 2011/12/20 00:00 [accepted]
PHST- 2012/01/27 06:00 [entrez]
PHST- 2012/01/27 06:00 [pubmed]
PHST- 2012/07/10 06:00 [medline]
PHST- 2012/01/18 00:00 [pmc-release]
AID - PONE-D-11-10856 [pii]
AID - 10.1371/journal.pone.0030430 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(1):e30430. doi: 10.1371/journal.pone.0030430. Epub 2012 Jan 18.