PMID- 22280954
OWN - NLM
STAT- MEDLINE
DCOM- 20120906
LR  - 20181201
IS  - 1090-2392 (Electronic)
IS  - 0011-2240 (Linking)
VI  - 64
IP  - 3
DP  - 2012 Jun
TI  - Effectiveness of human mesenchymal stem cells derived from bone marrow 
      cryopreserved for 23-25 years.
PG  - 167-75
LID - 10.1016/j.cryobiol.2012.01.004 [doi]
AB  - OBJECTIVE: To evaluate long-term cryopreserved human bone marrow cells (BMCs) as 
      a source of functional mesenchymal stem cells (MSCs). METHODS: Samples of human 
      BMCs that were cryopreserved for 23-25 years (n=20) were thawed to obtain an 
      initial culture and a primary culture (P(0)) that was propagated through five 
      passages (P(1)-P(5)) to obtain MSCs. Freshly collected human bone marrow samples 
      (n=20) were used as controls for comparison of efficiency of recovery and growth 
      characteristics of MSCs. P(3) cultures were tested for their capacity to 
      differentiate into osteoblasts, adipocytes, and neuronal cells. Appropriate 
      staining, immunohistochemical and biochemical methods were employed to ascertain 
      cell type identities at different stages of culturing. RESULTS: In the initial 
      culture, the cell adherence rate of the cryopreserved cells was significantly 
      lower than that of controls (19.7% vs. 38.2%, p<0.05) while the relative rate of 
      recovery of MSCs was only 48.5+/-8.6% in P(0). At the end of P(3), fibroblast-like 
      cells accounted for about 95% of cells in both cryopreserved and control groups 
      (p>0.05). These cells were positive for essential MSC surface molecules (CD90, 
      CD105, CD166, CD44, CD29, CD71, CD73) and negative for haematopoietic and 
      endothelial cell markers (CD45, CD34, HLA-DR). The cell growth and cell cycle 
      patterns were similar for both groups. MSCs at P(3) from both groups had similar 
      capacities to differentiate in vitro into osteoblasts, adipocytes, and neuronal 
      cells. CONCLUSION: Using the methods described here, long-term (23-25 years) 
      cryopreserved human BMCs can be successfully cultivated to obtain MSCs that have 
      good differentiation capabilities.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Shen, Jian-liang
AU  - Shen JL
AD  - Department of Haematology, PLA Navy General Hospital, Beijing 100048, China. 
      shenjianliang@csco.org.cn
FAU - Huang, You-zhang
AU  - Huang YZ
FAU - Xu, Shi-xia
AU  - Xu SX
FAU - Zheng, Pei-hao
AU  - Zheng PH
FAU - Yin, Wen-jie
AU  - Yin WJ
FAU - Cen, Jian
AU  - Cen J
FAU - Gong, Li-zhong
AU  - Gong LZ
LA  - eng
PT  - Journal Article
DEP - 20120118
PL  - Netherlands
TA  - Cryobiology
JT  - Cryobiology
JID - 0006252
RN  - 0 (Antigens, CD)
RN  - 0 (Cryoprotective Agents)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - Adipocytes/cytology
MH  - Antigens, CD/analysis
MH  - Biological Specimen Banks/*statistics & numerical data
MH  - Bone Marrow Cells/*cytology/drug effects
MH  - Cell Adhesion/drug effects
MH  - Cell Count
MH  - Cell Cycle/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - *Cryopreservation
MH  - Cryoprotective Agents/pharmacology
MH  - Dimethyl Sulfoxide/pharmacology
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - Mesenchymal Stem Cells/*cytology/drug effects
MH  - Neurons/cytology
MH  - Osteoblasts/cytology
MH  - Time Factors
EDAT- 2012/01/28 06:00
MHDA- 2012/09/07 06:00
CRDT- 2012/01/28 06:00
PHST- 2011/06/29 00:00 [received]
PHST- 2011/12/15 00:00 [revised]
PHST- 2012/01/09 00:00 [accepted]
PHST- 2012/01/28 06:00 [entrez]
PHST- 2012/01/28 06:00 [pubmed]
PHST- 2012/09/07 06:00 [medline]
AID - S0011-2240(12)00005-3 [pii]
AID - 10.1016/j.cryobiol.2012.01.004 [doi]
PST - ppublish
SO  - Cryobiology. 2012 Jun;64(3):167-75. doi: 10.1016/j.cryobiol.2012.01.004. Epub 
      2012 Jan 18.