PMID- 22282416
OWN - NLM
STAT- MEDLINE
DCOM- 20120521
LR  - 20120127
IS  - 1615-9314 (Electronic)
IS  - 1615-9306 (Linking)
VI  - 35
IP  - 4
DP  - 2012 Feb
TI  - Quantification of gamma-aminobutyric acid in the heads of houseflies (Musca 
      domestica) and diamondback moths (Plutella xylostella (L.)), using capillary 
      electrophoresis with laser-induced fluorescence detection.
PG  - 548-55
LID - 10.1002/jssc.201100802 [doi]
AB  - A novel method was developed for quantifying the levels of gamma-aminobutyric acid 
      (GABA) in the heads of houseflies (Musca domestica) and diamondback moths 
      (Plutella xylostella (L.)), using capillary electrophoresis with laser-induced 
      fluorescence detection (CE-LIF). The GABA in sample was derivatized with 
      4-chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl) prior to CE-LIF analysis. In 
      total, 32 mmol/L borate buffer, at pH 9.2 and containing 5.3 mmol/L 
      beta-cyclodextrin (beta-CD) and 10.4 mmol/L sodium dodecyl sulfate (SDS), was 
      determined to be the optimum CE background electrolyte (BGE) for GABA analysis. 
      The detection limit of GABA was 0.016 mumol/L. The relative standard deviations 
      (RSDs) of the migration time and peak area of GABA were 1.78 and 4.93%, 
      respectively. The average recoveries of 0.97, 3.88, and 5.83 mumol/L of GABA, each 
      added to the head sample of housefly, ranged from 88.9 to 110.5%. This method is 
      simple and applicable to GABA assays of the heads of insects. With this newly 
      developed CE-LIF method, the amounts of GABA in the heads of houseflies (M. 
      domestica) and diamondback moths (P. xylostella (L.)) were measured. The results 
      are relevant to the understandings of some insecticides and 
      insecticide-resistance mechanisms in pests.
CI  - Copyright (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Shi, Xueyan
AU  - Shi X
AD  - Department of Entomology, China Agricultural University, Beijing, PR China. 
      shixueyan@cau.edu.cn
FAU - Liang, Pei
AU  - Liang P
FAU - Song, Dunlun
AU  - Song D
FAU - Yang, Wenling
AU  - Yang W
FAU - Gao, Xiwu
AU  - Gao X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Sep Sci
JT  - Journal of separation science
JID - 101088554
RN  - 56-12-2 (gamma-Aminobutyric Acid)
SB  - IM
MH  - Animals
MH  - Electrophoresis, Capillary
MH  - Fluorescence
MH  - *Head
MH  - Houseflies/*anatomy & histology
MH  - Lasers
MH  - Luminescent Measurements/*methods
MH  - Moths/*anatomy & histology
MH  - gamma-Aminobutyric Acid/*analysis
EDAT- 2012/01/28 06:00
MHDA- 2012/05/23 06:00
CRDT- 2012/01/28 06:00
PHST- 2012/01/28 06:00 [entrez]
PHST- 2012/01/28 06:00 [pubmed]
PHST- 2012/05/23 06:00 [medline]
AID - 10.1002/jssc.201100802 [doi]
PST - ppublish
SO  - J Sep Sci. 2012 Feb;35(4):548-55. doi: 10.1002/jssc.201100802.