PMID- 22284121
OWN - NLM
STAT- MEDLINE
DCOM- 20120517
LR  - 20211021
IS  - 1742-4690 (Electronic)
IS  - 1742-4690 (Linking)
VI  - 9
DP  - 2012 Jan 27
TI  - Bone marrow stromal cell antigen 2 (BST-2) restricts mouse mammary tumor virus 
      (MMTV) replication in vivo.
PG  - 10
LID - 10.1186/1742-4690-9-10 [doi]
AB  - BACKGROUND: Bone marrow stromal cell antigen 2 (BST-2) is a cellular factor that 
      restricts the egress of viruses such as human immunodeficiency virus (HIV-1) from 
      the surface of infected cells, preventing infection of new cells. BST-2 is 
      variably expressed in most cell types, and its expression is enhanced by 
      cytokines such as type I interferon alpha (IFN-alpha). In this present study, we used 
      the beta-retrovirus, mouse mammary tumor virus (MMTV) as a model to examine the 
      role of mouse BST-2 in host infection in vivo. RESULTS: By using RNA 
      interference, we show that loss of BST-2 enhances MMTV replication in cultured 
      mammary tumor cells and in vivo. In cultured cells, BST-2 inhibits virus 
      accumulation in the culture medium, and co-localizes at the cell surface with 
      virus structural proteins. Furthermore, both scanning electron micrograph (SEM) 
      and transmission electron micrograph (TEM) show that MMTV accumulates on the 
      surface of IFNalpha-stimulated cells. CONCLUSIONS: Our data provide evidence that 
      BST-2 restricts MMTV release from naturally infected cells and that BST-2 is an 
      antiviral factor in vivo.
FAU - Jones, Philip H
AU  - Jones PH
AD  - Department of Microbiology, University of Iowa, Carver College of Medicine, Iowa 
      City, IA, USA.
FAU - Mehta, Harshini V
AU  - Mehta HV
FAU - Maric, Martina
AU  - Maric M
FAU - Roller, Richard J
AU  - Roller RJ
FAU - Okeoma, Chioma M
AU  - Okeoma CM
LA  - eng
GR  - K22 AI081899/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120127
PL  - England
TA  - Retrovirology
JT  - Retrovirology
JID - 101216893
RN  - 0 (Antigens, CD)
RN  - 0 (BST2 protein, mouse)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*metabolism
MH  - Cell Line
MH  - Gene Silencing
MH  - Mammary Tumor Virus, Mouse/*immunology/*physiology
MH  - Membrane Glycoproteins/*metabolism
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - RNA Interference
MH  - *Virus Release
PMC - PMC3283513
EDAT- 2012/01/31 06:00
MHDA- 2012/05/18 06:00
PMCR- 2012/01/27
CRDT- 2012/01/31 06:00
PHST- 2011/10/20 00:00 [received]
PHST- 2012/01/27 00:00 [accepted]
PHST- 2012/01/31 06:00 [entrez]
PHST- 2012/01/31 06:00 [pubmed]
PHST- 2012/05/18 06:00 [medline]
PHST- 2012/01/27 00:00 [pmc-release]
AID - 1742-4690-9-10 [pii]
AID - 10.1186/1742-4690-9-10 [doi]
PST - epublish
SO  - Retrovirology. 2012 Jan 27;9:10. doi: 10.1186/1742-4690-9-10.