PMID- 22284485
OWN - NLM
STAT- MEDLINE
DCOM- 20120622
LR  - 20171116
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 89
DP  - 2012 Jan 30
TI  - Determination of HT-2 and T-2 toxins in oats and wheat by ultra-performance 
      liquid chromatography with photodiode array detection.
PG  - 231-6
LID - 10.1016/j.talanta.2011.12.017 [doi]
AB  - European intake estimates indicate that the presence of HT-2 and T-2 toxins in 
      cereals, mainly in oats, can be of concern for human health. Therefore, the 
      development of sensitive, rapid and reliable methods for determining these 
      mycotoxins in cereals, in particular oats, has high priority. A rapid 
      ultra-performance liquid chromatographic (UPLC) method has been developed for the 
      simultaneous determination of HT-2 and T-2 toxins in oats and wheat at mug kg(-1) 
      level. Ground samples were extracted with methanol/water (90:10, v/v) and the 
      diluted extracts were cleaned up through immunoaffinity columns. HT-2 and T-2 
      toxins were separated and quantified by UPLC with photodiode array (PDA) detector 
      (lambda=202 nm) in less than 5 min. Mean recoveries from blank oats samples spiked 
      with HT-2 and T-2 toxins at levels of 50-1000 mug kg(-1) ranged from 87 to 96%, 
      with relative standard deviations (RSDs) lower than 7%; mean recoveries from 
      wheat spiked with HT-2 and T-2 toxins at levels of 25-100 mug kg(-1) ranged from 
      91 to 103%, with RSDs lower than 5%. The limit of detection of the method was 8 
      mug kg(-1) for both toxins (signal-to-noise ratio 3:1). The method was 
      successfully applied to the analysis of HT-2 and T-2 toxins in naturally 
      contaminated oats and wheat samples. A good correlation was found by comparative 
      analysis of naturally contaminated samples of oats (r=0.9985) and wheat 
      (r=0.9058) using the proposed method or a reliable HPLC method with fluorescence 
      detection after pre-column derivatization with 1-anthroylnitrile.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Pascale, Michelangelo
AU  - Pascale M
AD  - Institute of Sciences of Food Production (ISPA), National Research Council of 
      Italy (CNR), via G. Amendola 122/O, 70126 Bari, Italy. 
      michelangelo.pascale@ispa.cnr.it
FAU - Panzarini, Giuseppe
AU  - Panzarini G
FAU - Visconti, Angelo
AU  - Visconti A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111209
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Anthracenes)
RN  - 3752-42-9 (1-anthranoylnitrile)
RN  - I3FL5NM3MO (T-2 Toxin)
RN  - NC6C26RM46 (HT-2 toxin)
SB  - IM
MH  - Anthracenes
MH  - Avena/*chemistry
MH  - Chromatography, Affinity
MH  - Chromatography, High Pressure Liquid
MH  - Food Contamination/analysis
MH  - Limit of Detection
MH  - Signal-To-Noise Ratio
MH  - Spectrometry, Fluorescence
MH  - T-2 Toxin/*analogs & derivatives/*analysis
MH  - Triticum/*chemistry
EDAT- 2012/01/31 06:00
MHDA- 2012/06/23 06:00
CRDT- 2012/01/31 06:00
PHST- 2011/08/30 00:00 [received]
PHST- 2011/12/02 00:00 [revised]
PHST- 2011/12/06 00:00 [accepted]
PHST- 2012/01/31 06:00 [entrez]
PHST- 2012/01/31 06:00 [pubmed]
PHST- 2012/06/23 06:00 [medline]
AID - S0039-9140(11)01085-X [pii]
AID - 10.1016/j.talanta.2011.12.017 [doi]
PST - ppublish
SO  - Talanta. 2012 Jan 30;89:231-6. doi: 10.1016/j.talanta.2011.12.017. Epub 2011 Dec 
      9.