PMID- 22285730
OWN - NLM
STAT- MEDLINE
DCOM- 20120710
LR  - 20120224
IS  - 1745-7270 (Electronic)
IS  - 1672-9145 (Linking)
VI  - 44
IP  - 3
DP  - 2012 Mar
TI  - Low microRNA-199a expression in human amniotic epithelial cell feeder layers 
      maintains human-induced pluripotent stem cell pluripotency via increased leukemia 
      inhibitory factor expression.
PG  - 197-206
LID - 10.1093/abbs/gmr127 [doi]
AB  - Human-induced pluripotent stem (iPS) cells share the same key properties as 
      embryonic stem cells, and may be generated from patient- or disease-specific 
      sources, which makes them attractive for personalized medicine, drug screens, or 
      cellular therapy. Long-term cultivation and maintenance of normal iPS cells in an 
      undifferentiated self-renewing state is a major challenge. Our previous studies 
      have shown that human amniotic epithelial cells (HuAECs) could provide a good 
      source of feeder cells for mouse and human embryonic stem cells, or 
      spermatogonial stem cells, as they express endogenous leukemia inhibitory factor 
      (LIF) at high levels. Here, we examined the effect of exogenous microRNA-199a 
      regulation on endogenous LIF expression in HuAECs, and in turn on human iPS cell 
      pluripotency. We found that HuAECs feeder cells transfected with microRNA-199a 
      mutant expressed LIF at high levels, allowing iPS to maintain a high level of 
      alkaline phosphatase activity in long-term culture and form teratomas in severe 
      combined immunodeficient mice. The expression of stem cell markers was increased 
      in iPS cultured on HuAECs feeder cells transfected with the microRNA-199a mutant, 
      compared with iPS cultured on HuAECs transfected with microRNA-199a or mouse 
      embryo fibroblasts. Taken together, these results suggested that LIF expression 
      might be regulated by microRNA-199a, and LIF was a crucial component in feeder 
      cells, and also was required for maintenance of human iPS cells in an 
      undifferentiated, proliferative state capable of self-renewal.
FAU - Liu, Te
AU  - Liu T
AD  - School of Environmental Science and Engineering, Donghua University, Shanghai, 
      China. liute79@yahoo.com
FAU - Chen, Qing
AU  - Chen Q
FAU - Huang, Yongyi
AU  - Huang Y
FAU - Huang, Qin
AU  - Huang Q
FAU - Jiang, Lizhen
AU  - Jiang L
FAU - Guo, Lihe
AU  - Guo L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120126
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (Leukemia Inhibitory Factor)
RN  - 0 (MicroRNAs)
RN  - 0 (mirn199 microRNA, human)
SB  - IM
MH  - Amniotic Fluid/*cytology
MH  - Animals
MH  - Cell Culture Techniques/*methods
MH  - Coculture Techniques/methods
MH  - Culture Techniques/*methods
MH  - Epithelial Cells/*cytology
MH  - Fibroblasts/cytology
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Leukemia Inhibitory Factor/*biosynthesis/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*biosynthesis/physiology
MH  - Pluripotent Stem Cells/*cytology
MH  - Stem Cells/cytology
EDAT- 2012/01/31 06:00
MHDA- 2012/07/11 06:00
CRDT- 2012/01/31 06:00
PHST- 2012/01/31 06:00 [entrez]
PHST- 2012/01/31 06:00 [pubmed]
PHST- 2012/07/11 06:00 [medline]
AID - gmr127 [pii]
AID - 10.1093/abbs/gmr127 [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2012 Mar;44(3):197-206. doi: 
      10.1093/abbs/gmr127. Epub 2012 Jan 26.