PMID- 22285951
OWN - NLM
STAT- MEDLINE
DCOM- 20120511
LR  - 20120308
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 136
IP  - 2
DP  - 2012
TI  - Molecular characterization and physical mapping of two classes of 5S rDNA in the 
      genomes of Gymnotus sylvius and G. inaequilabiatus (Gymnotiformes, Gymnotidae).
PG  - 131-7
LID - 10.1159/000335658 [doi]
AB  - The nucleotide sequences of the 5S rRNA multigene family and their distribution 
      across the karyotypes in 2 species of Gymnotiformes, genus Gymnotus (G. sylvius 
      and G. inaequilabiatus) were investigated by means of fluorescence in situ 
      hybridization (FISH). The results showed the existence of 2 distinct classes of 
      5S rDNA sequences in both species: class I and class II. A high conservative 
      pattern of the codifying region of the 5S rRNA gene was identified, contrasting 
      with significant alterations detected in the nontranscribed spacer (NTS). The 
      presence of TATA-like sequences along the NTS of both species was an expected 
      occurrence, since such sequences have been associated with the regulation of the 
      gene expression. FISH using 5S rDNA class I and class II probes revealed that 
      both gene classes were collocated in the same chromosome pair in the genome of G. 
      sylvius, while in that of G. inaequilabiatus, class II appeared more disperse 
      than class I.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - Scacchetti, P C
AU  - Scacchetti PC
AD  - Instituto de Biociencias de Botucatu, Departamento de Morfologia, Universidade 
      Estadual Paulista, Sao Paulo, Brazil. pcardim@ibb.unesp.br
FAU - Alves, J C P
AU  - Alves JC
FAU - Utsunomia, R
AU  - Utsunomia R
FAU - Claro, F L
AU  - Claro FL
FAU - de Almeida Toledo, L F
AU  - de Almeida Toledo LF
FAU - Oliveira, C
AU  - Oliveira C
FAU - Foresti, F
AU  - Foresti F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120125
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping/*methods
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Electrophoresis, Agar Gel
MH  - Genome/*genetics
MH  - Gymnotiformes/classification/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Karyotyping
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 5S/*genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
MH  - Species Specificity
EDAT- 2012/01/31 06:00
MHDA- 2012/05/12 06:00
CRDT- 2012/01/31 06:00
PHST- 2011/11/02 00:00 [accepted]
PHST- 2012/01/31 06:00 [entrez]
PHST- 2012/01/31 06:00 [pubmed]
PHST- 2012/05/12 06:00 [medline]
AID - 000335658 [pii]
AID - 10.1159/000335658 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2012;136(2):131-7. doi: 10.1159/000335658. Epub 2012 Jan 
      25.