PMID- 22286789
OWN - NLM
STAT- MEDLINE
DCOM- 20120405
LR  - 20220321
IS  - 0172-780X (Print)
IS  - 0172-780X (Linking)
VI  - 32
IP  - 6
DP  - 2011
TI  - Estrogen altered facial mechanical pain threshold and trigeminal P2X3 receptor 
      expression.
PG  - 811-5
AB  - OBJECTIVES: P2X3 receptors are expressed in trigeminal ganglia (TG) and 
      participate in the transduction of facial pain. However, the mechanisms 
      underlying P2X receptor-mediated nociception at different estrogen levels has not 
      been examined. METHODS: In this study, female rats were randomly divided into 
      sham-operated (sham), ovariectomized (OVX), and estrogen-treated groups. In each 
      group, the facial mechanical pain threshold was tested and the TG were harvested 
      for a real-time PCR analysis of P2X3 receptor mRNA and western blot analysis of 
      protein level. RESULTS: In OVX rats we found that the mechanical pain threshold 
      was significantly decreased compared with that in sham rats. Estrogen replacement 
      reversed the decrease. The expression of P2X3 mRNA level in TG from OVX rats was 
      significantly increased, consistent with the enhanced P2X3 receptor in protein 
      level. Estrogen replacement could decrease the expression of P2X3 receptor in 
      both mRNA and protein level. CONCLUSION: These results indicate that estrogen 
      might modulate the transduction of facial pain by inhibiting P2X3 receptor in TG.
FAU - Yu, Li-hua
AU  - Yu LH
AD  - Department of Physiology, The Key Laboratory of Molecular Neurobiology, Ministry 
      of Education, Second Military Medical University, Shanghai, P.R.China.
FAU - Li, Na
AU  - Li N
FAU - Liu, Cai-yue
AU  - Liu CY
FAU - Ma, Bei
AU  - Ma B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Sweden
TA  - Neuro Endocrinol Lett
JT  - Neuro endocrinology letters
JID - 8008373
RN  - 0 (Estrogens)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Purinergic P2X3)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Behavior, Animal/physiology
MH  - Estradiol/pharmacology
MH  - Estrogens/pharmacology/*physiology
MH  - Facial Pain/*physiopathology
MH  - Female
MH  - Male
MH  - Ovariectomy
MH  - Pain Measurement
MH  - Pain Threshold/*drug effects
MH  - RNA, Messenger/metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Purinergic P2X3/genetics/*metabolism
MH  - Trigeminal Ganglion/cytology/*metabolism
EDAT- 2012/01/31 06:00
MHDA- 2012/04/06 06:00
CRDT- 2012/01/31 06:00
PHST- 2009/11/04 00:00 [received]
PHST- 2011/09/15 00:00 [accepted]
PHST- 2012/01/31 06:00 [entrez]
PHST- 2012/01/31 06:00 [pubmed]
PHST- 2012/04/06 06:00 [medline]
AID - NEL320611A22 [pii]
PST - ppublish
SO  - Neuro Endocrinol Lett. 2011;32(6):811-5.